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The response of tartary buckwheat and 19 bZIP genes to abscisic acid (ABA)

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Abstract

Tartary buckwheat is a kind of plant which can be used as medicine as well as edible. Abscisic acid (ABA) signaling plays an important role in the response of plants such as tartary buckwheat to drought and other stress. However, there are not many studies on tartary buckwheat by ABA treatment. In this study, the germination, root length, stoma, and anthocyanin accumulation of tartary buckwheat were all significantly affected by ABA. ABA signaling is important for plants to respond to drought and other stresses, the bZIP gene family is an important member of the ABA signaling pathway. Through the analysis of the origin relationship between tartary buckwheat bZIP family and its related species, 19 bZIP genes in tartary buckwheat were found to be relatively conserved, which laid a foundation for further study of bZIP family. The qRT-PCR results showed that most of the group members were induced by ABA treatment, including 0, 15, 30, 50, 70 µM ABA and 0, 0.5, 2, 4, 8, 16, 24 h ABA treatment. These results suggested that ABA could affect the growth and development of tartary buckwheat, and FtbZIPs might have different functions in the response of tartary buckwheat to drought. This study will be helpful to further analyze the genetic breeding and economic value of tartary buckwheat resistance.

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Acknowledgements

This research was supported by grants from the National Transgene Project (2016ZX08009003-002 to J.W.), the Nation Natural Science Foundation of China (31870240 to Y. Y.), Sichuan Wheat Crop Breeding Project (2016nyz003 to A. W.), and Sichuan Wheat Innovation Team (2016SCYZ02 to A. W.).

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Author notes

  1. Shuya **ao and Yu Liu have contributed equally to this study and regarded as co-first authors.

Authors and Affiliations

  1. Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, China

    Shuya **ao, Yu Liu, Yaodong Liu, **aoyi Li, Zhibin Liu, Xufeng Li, Yi Yang & Jianmei Wang

  2. **chang College, **chang, 615000, China

    Anhu Wang

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  1. Shuya **ao
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Contributions

SX performed the experiments and wrote the manuscript; YL contributed significantly to the experiments and data analysis; AW provided the seeds of Tartary buckwheat; YL provided the analysis of bioinformatics; JW contributed to the conception of the study; others assisted with the experiments.

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Correspondence to Jianmei Wang.

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**ao, S., Liu, Y., Wang, A. et al. The response of tartary buckwheat and 19 bZIP genes to abscisic acid (ABA). Mol Biol Rep 48, 4341–4350 (2021). https://doi.org/10.1007/s11033-021-06449-z

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  • DOI: https://doi.org/10.1007/s11033-021-06449-z

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