Abstract
Engrailed-2 (EN2), a member of the homeobox family of genes, encodes a homeodomain-containing transcription factor that is thought to be a potential oncogene in a number of cancers. Because the role of EN2 in clear cell renal cell carcinoma (CCRCC) has not been determined, we investigated its expression in CCRCC tissues and cell lines. Using immunohistochemical (IHC) staining, we found that EN2 protein was expressed in normal renal cells and tubules, but was frequently down-regulated in tissues from patients with CCRCC and in CCRCC cell lines. In addition, we found that EN2, which functions in the nucleus, was completely localized to the cytoplasm of CCRCC cells as detected by IHC and immunofluorescence staining. Furthermore, expression of EN2 protein was negatively correlated with increasing histological grade of CCRCC tumors (P = 0.003). The exact role of EN2 expression in renal carcinoma carcinogenesis requires further investigation.
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Abbreviations
- CCRCC:
-
Clear cell renal cell carcinoma
- EN2:
-
Engrailed 2
- RT-qPCR:
-
Real-time quantitative polymerase chain reaction
- IHC:
-
Immunohistochemistry
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Acknowledgments
The authors wish to thank Hong Li for help in sample collection as well as Professor Guang-yu Jiang, Dr Bin Du, and Prof Huan Zhang for technical assistance. We thank our patients for their support and cooperation for this study. This study was supported by the Key specialist of Guangdong Provincial 12th Five-Year Plan (No. 201220) and Guangzhou Municipal Health Bureau Grant (N0. 20121A011159).
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Lai, Cy., Pan, B., Luo, Y. et al. Engrailed-2 is down-regulated but also ectopically expressed in clear cell renal cell carcinoma. Mol Biol Rep 41, 3651–3657 (2014). https://doi.org/10.1007/s11033-014-3229-z
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DOI: https://doi.org/10.1007/s11033-014-3229-z