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Modulation of hsa-miR-26b levels following adipokine stimulation

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Abstract

MicroRNAs (miRNAs) are short non-coding RNAs that are involved in post-transcriptional regulation of gene expression. Hsa-miR-26b is an intronic miRNA located in the intron of CTDSP1 (carboxy-terminal domain, RNA polymerase II, polypeptide A, small phosphatase 1). In the present study, the expression of hsa-miR-26b was examined during human preadipocyte differentiation. 15 days after induction of differentiation, mature human adipocytes were treated with adipokines. Hsa-miR-26b was differentially expressed during human preadipocyte differentiation. Tumor necrosis factor-α (TNF-α), leptin and resistin, but not interleukin-6, caused downregulation of hsa-miR-26b expression in adipocytes. These results suggest that the expression of hsa-miR-26b is affected by TNF-α, leptin and resistin and that hsa-miR-26b may be an important mediator in regulating the obesity-related insulin sensitivity and inflammatory responses.

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Acknowledgments

This project was supported by grants from the National Key Basic Research Program of China (2013CB530604), the National Natural Science Foundation of China (No. 81170797), the Natural Science Foundation of Jiangsu Province China (BK2011107), the Program for Innovative Research Teams of Jiangsu Province (LJ201108), and Nan**g Technological Development Program (201104013).

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No competing financial interests exist.

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Authors and Affiliations

  1. Department of Laboratory Medicine, The 82nd Hospital of the People’s Liberation Army, No.100 Jiankang East Road, Huaian, 223001, China

    Guangfeng Xu, Hailong Fu & Ya** Zhao

  2. State Key Laboratory of Reproductive Medicine, Department of Children Health Care, Nan**g Maternity and Child Health Care Hospital Affiliated to Nan**g Medical University, No. 123 Tianfei Road, Nan**g, 210029, China

    Guangfeng Xu, Chenbo Ji & **rong Guo

  3. Institute of Pediatrics, Nan**g Medical University, Nan**g, 210029, China

    Chunmei Shi, Lingling Zhu, Lu Zhu, Lulian Xu, Ling Chen & Yueying Feng

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  1. Guangfeng Xu
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  2. Chenbo Ji
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  3. Chunmei Shi
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  9. Yueying Feng
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  10. Ya** Zhao
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  11. **rong Guo
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Corresponding authors

Correspondence to Ya** Zhao or **rong Guo.

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Guangfeng Xu and Chenbo Ji contributed equally to this study.

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Xu, G., Ji, C., Shi, C. et al. Modulation of hsa-miR-26b levels following adipokine stimulation. Mol Biol Rep 40, 3577–3582 (2013). https://doi.org/10.1007/s11033-012-2431-0

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  • DOI: https://doi.org/10.1007/s11033-012-2431-0

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