Abstract
Lanthanum Zirconate nanoparticles (NPs) are used in blades of gas turbine engines to thermally insulate them and to protect them against hot and corrosive gas streams. However, the information regarding their biocompatibility is limited. The present study was aimed to report the effect of Lanthanum Zirconate NPs on selected aspects of behavior, serum biochemistry, complete blood count and antioxidant parameters from vital organs of albino mice in a gender specific manner. Albino mice, seven weeks old, were orally treated with 75 mg/ml solvent/Kg body weight of Lanthanum Zirconate nanoparticles for consecutive 22 days. Saline treated control groups were maintained in parallel. It was observed that rearing frequency was significantly decreased (P = 0.01) in NPs treated male mice. Complete blood count analysis indicated that NPs treated female mice had significantly reduced white blood cells (P = 0.05) and lymphocytes count (P = 0.03). NPs treated male had significantly reduced serum cholesterol levels (P = 0.05) than control group. It was observed that Superoxide dismutase concentrations in liver (P = 0.025) and kidney (P = 0.008), Malondialdehyde concentrations in liver (P = 0.044) of female and Malondialdehyde concentrations in kidney (P < 0.001) and brain (P < 0.001) and catalase concentrations in liver (P = 0.05) of NPs treated male mice were significantly higher than their respective control groups.. In conclusion, we are reporting that oral supplementation with 75 mg/ml solvent/Kg body weight of Lanthanum Zirconate nanoparticles can affect the behavior, leukocyte count, serum cholesterol and antioxidant metabolites from vital organs of albino mice in a gender specific manner.
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Supplementary Fig. 1
Comparison of Rota rod test results between saline treated control and albino mice treated with 75 mg/ml solvent/Kg body weight of Lanthanum Zirconate nanoparticles for 22 days. N = 10 for each treatment. All values are expressed as mean ± slandered error of mean. P-value represents the results of 2 sample t-test. (JPG 56 kb)
Supplementary Fig 2
Comparison of gain in body weight for 22 days on daily basis between Lanthnum Zirconate nanoparticles treated (75 mg/ml of solvent/Kg body weight) and untreated adult albino mice of both genders. N = 10 for each treatment. Data is expressed as mean ± standard error of mean. P > 0.05 = Non significant for all each day (2 sample t test) (JPG 57 kb)
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Aftab, M.N., Akram, I.N., Khosa, T. et al. Oral supplementation of Lanthanum Zirconate nanoparticles moderately affected behavior but drastically disturbed leukocyte count, serum cholesterol levels and antioxidant parameters from vital organs of albino mice in a gender specific manner. Metab Brain Dis 33, 1421–1429 (2018). https://doi.org/10.1007/s11011-018-0248-9
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DOI: https://doi.org/10.1007/s11011-018-0248-9