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Analysis of time-course gene expression profiles of sinusoidal endothelial cells during liver regeneration in rats

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Abstract

Liver regeneration (LR) after partial hepatectomy (PH) requires the coordinate contribution of different cell types. Liver sinusoidal endothelial cells (LSECs), representing the largest population of nonparenchymal cells, are proven to be crucial in LR. However, the details about their implications in regeneration are not still clear. In this study, percoll density centrifugation and immunomagentic bead methods were used to isolate LSECs with high purity and yield; global transcriptional profiles of LSECs during the regeneration were investigated by microarray. 1,629 genes were identified to be LR-related. Among them, there were 833 known genes whose expression patterns were clustered into eight classes. Gene function enrichment analysis showed that genes involved in the major LSEC functions, i.e., coagulation, phagocytosis, and transport, were highly enriched in cluster characterized by rapid induction and gradual return, suggesting the quick reestablishment of LSEC function after PH. Genes in immunity/inflammation and defense response were enriched in clusters exhibiting transient downregulation and quick recovery, possibly being associated with suppression of immunity/inflammation pathway in LSECs at early phase. Genes in glycogen synthesis and glycolysis were enriched in the clusters marked by “significant increase and gradual return” and “slight increase and then downregulation”, implying an enhanced carbohydrate metabolism at early phase; detoxification-related genes were markedly distributed in the cluster with feature of rapid increase and then reduction, which was helpful in eliminating waste substance. Taken together, the measurement of gene expression profiling of LSECs and expression pattern analysis of functionally categorized genes gave insight into the mechanism of action of this cell on LR.

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Acknowledgments

This work was supported by the National Basic Research 973 Pre-research Program of China (No. 2010CB534905)

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Authors and Affiliations

  1. College of Life Science, Henan Normal University, 46# East of Construction Road, ** Wang

  2. Key Laboratory for Cell Differentiation Regulation, Henan Normal University, 46# East of Construction Road, ** Wang, Wen-bo Wang, Lian-xing Zhang, Qiu-shi Zhu & Lei Wang

  3. College of Life Science and Technology, **njiang University, Urmuqi, 830046, China

    **ao-guang Chen & Cui-fang Chang

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  1. Cun-shuan Xu
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  2. **ao-guang Chen
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  4. Gai-** Wang
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  8. Lei Wang
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Correspondence to Cun-shuan Xu.

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Xu, Cs., Chen, Xg., Chang, Cf. et al. Analysis of time-course gene expression profiles of sinusoidal endothelial cells during liver regeneration in rats. Mol Cell Biochem 350, 215–227 (2011). https://doi.org/10.1007/s11010-010-0701-5

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  • DOI: https://doi.org/10.1007/s11010-010-0701-5

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