Abstract
Approximately, 10% of the world population is facing the challenge of food allergy in direct or indirect way. In this study, a genome-wide identification and annotation of the novel putative allergen from almond is performed. Initially, the whole proteome of almond (31,000 proteins) was scanned by Allergenonline, a publically available database of already reported allergens from different sources. The detailed analysis suggests that there are 430 putative allergens which reduced to 45 on motif-based screening using AllFam database. These predicted allergens are annotated for their function by using PFAM, GO databases and orthology analysis. To validate our prediction, we have used structural insights of allergen and antibody interactions for one of the predicted putative allergen protein, homologous to Pru ar 3.0101allergen from apricot. The structure of putative allergen was modeled and molecular docking studies were performed against the antibody. The best docked conformation was subjected to molecular simulation studies to confirm the stable binding of these two molecules. This detailed analysis suggests that the identified allergen will show cross reactivity similar to Pru ar 3.0101 allergen from apricot. This is one of the first report of identifying and annotating the homologous of Pru ar 3.0101 allergen in almond.
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References
Alioto T et al (2020) Transposons played a major role in the diversification between the closely related almond and peach genomes: results from the almond genome sequence. Plant J 101:455–472
Andorf S et al (2017) Association of clinical reactivity with sensitization to allergen components in multifood-allergic children. J Allergy Clin Immunol Pract 5(5):1325–34
Che H, Z Y (2019) Identification of almond (Prunus dulcis) vicilin as a food allergen. J Agric Food Chem 6(1):425–432
Dereeper A, A S et al (2010) BLAST-EXPLORER helps you building datasets for phylogenetic analysis. BMC Evol Biol 10(1):1–6
Elizur A (2018) NUT co reactivity - acquiring knowledge for elimination recommendations (NUT CRACKER) study. Allergy Eur J Allergy Clin Immunol 73:593–601
Flores E, C L L (2012) Plant food allergy in patients with pollinosis from the mediterranean area. Int Arch Allergy Immunol 159(4):346–354
Gupta RS, W. C (2018) The public health impact of parent-reported childhood food allergies in the United States. Pediatrics 142:e20181235
Hofmann C, S. S, (2013) Cor a 1-reactive Tcells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen-associated food allergy to hazelnut. J Allergy Clin Immunol 131(5):1384–92
Huang JR (2016) CHARMM36m: an improved force field for folded and intrinsically disordered proteins. Nat Methods 14:71–73
Iweala OC (2018) Food allergy. Curr Gastroenterol Rep 20:17
LÁ E-Z (2020) TREE NUTS ALLERGY: knowledge, gaps and practical implications. Allergol Immunopathol (Madr) 48(4):313–315
Lechner MF et al (2011) Proteinortho: detection of (Co-)orthologs in large-scale analysis. BMC Bioinform 12(1):1–9
Lemkul J (2018) From Proteins to Perturbed Hamiltonians: A Suite of Tutorials for the GROMACS-2018 Molecular Simulation Package, v1.0. Living J Comp Mol Sci
McWilliam V et al (2019) Patterns of tree nut sensitization and allergy in the first 6 years of life in a population-based cohort. J Allergy Clin Immunol 143:644–650
Mittag D, A J (2004) Ara h 8, a Bet v 1-homologous allergen from peanut, is a major allergen in patients with combined birch pollen and peanut allergy. J Allergy Clin Immunol 114(6):1410–1417
Moore LE, Stewart PH, deShazo RD (2017) Food allergy: what we know now. Am J Med Sci 353:353–366
Mothes-Luksch N, R M (2017) Pru p 3, a marker allergen for lipid transfer protein sensitization also in Central Europe. Allergy 72(9):1415–1418
Mustafa SS, V. K (2020) Real-world tree nut consumption in peanut-allergic individuals. Ann Allergy Asthma Immunol 124:277–282
Pastorello EA (2004) Lipid transfer protein and vicilin are important walnut allergens in patients not allergic to pollen. J Allergy Clin Immunol 114(4):908–914
Pepper AN (2020) Consensus report from the food allergy research & education (FARE) 2019 oral immunotherapy for food allergy summit. J Allergy Clin Immunol 146(2):244–249
Radauer C (2017) Navigating through the jungle of allergens: features and applications of allergen databases. Int Arch Allergy Immunol 173:1–11
RC A (2000) Structural biology of allergens. J Allergy Clin Immunol 106(2):228–38
Rehm CD, Drewnowski A (2017) Replacing American snacks with tree nuts increases consumption of key nutrients among US children and adults: results of an NHANES modeling study. Nutr J 16(1):1–15
Sasaki M et al (2018) Prevalence of clinic-defined food allergy in early adolescence: the SchoolNuts study. J Allergy Clin Immunol 141:391–398
Singh BA (2022) Structural insights into the amino acid usage variations in the profilin gene family. Amino Acids 54(3):411–419
Smeekens JM, B K et al (2018) Tree nut allergies: allergen homology, cross-reactivity, and implications for therapy. Clin Exp Allergy 48(7):762–772
Tordesillas L, G-C C et al (2013) Transport of Pru p 3 across gastrointestinal epithelium an essential step towards the induction of food allergy? Clin Exp Allergy 43(12):1374–1383
Valenta R (1992) D. M. Profilins constitute a novel family of functional plant panallergens. J Exp Med. 175(2): 377 – 85
Wangorsch A, J. A.-W (2017) Identification and implication of an allergenic PR-10 protein from walnut in birch pollen associated walnut allergy. Mol Nutr Food Res.
Waterhouse AB (2018) SWISS-MODEL: homology modelling of protein structures and complexes. Nucleic Acids Res. W296-W303
Weinberger T, S. S (2018) Current perspectives on tree nut allergy: a review. J Asthma Allergy 11:41–51
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Singh, A., Upadhyay, A.K. Identification and Annotation of Peptide Allergens in Prunus dulcis. Int J Pept Res Ther 28, 157 (2022). https://doi.org/10.1007/s10989-022-10468-0
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DOI: https://doi.org/10.1007/s10989-022-10468-0