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Microcalorimetric investigation of five Aconitum L. plants on the metabolic activity of mitochondria isolated from rat liver

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Abstract

The fact that mitochondria still have metabolic activity and can live for a week with appropriate nutrients after isolated from the rat liver provides us a useful means to investigate the metabolic process of mitochondria influenced by chemicals. Using the TAM air isothermal microcalorimeter, the HP-time curves of the metabolic activity of mitochondria were measured. The effects of five Aconitum L. plants, Radix Aconiti Lateralis Preparata (RALP), Radix Aconiti (RA), Radix Aconiti Kusnezoffii (RAK), Radix Aconiti Brachypodi (RAB), and Radix Aconiti Singularis (RAS) on the metabolic activity of mitochondria were investigated, respectively. The quantitative information, such as k, Q, P max, T max, T lag, and P av obtained from the HP-time curves, were accumulated for analysis. With the help of PCA method, P av was selected as the standard for comparing the different effects of five Aconitum L. plants on metabolic activity of mitochondria. The potential sequence of efficiency was RAS > RAK > RAB > RA > RALP, and the EC50 were separately 1.43, 2.31, 1.10, 1.61, and 2.56 mg mL−1. The results demonstrated that the five Aconitum L. plants had different bioeffects on the mitochondria metabolism. Meanwhile, this study indicated that microcalorimetry was a powerful tool to evaluate the drugs’ efficiency on living system, providing some useful references for the application of five Aconitum L. plants in practice.

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Abbreviations

CHM:

Chinese herb medicine

RALP:

Radix Aconiti Lateralis Preparata

RA:

Radix Aconiti

RAK:

Radix Aconiti Kusnezoffii

RAB:

Radix Aconiti Brachypodi

RAS:

Radix Aconiti Singularis

PCA:

Principal component analysis

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Acknowledgements

We are grateful for the support of the National Natural Sciences Foundation (No. 81173571), National Basic Research Program of China (No. 2007CB512607), and the Major Projects of the National Science and Technology (2012ZX10005010-002-002).

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Authors and Affiliations

  1. China Military Institute of Chinese Medicine, 302 Hospital of People’s Liberation Army, 100, the 4th Ring Road, Bei**g, 100039, China

    Quanfu Zheng, Chunyu Li, Yanling Zhao, Jiabo Wang, Yaming Zhang & **aohe **ao

  2. College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China

    Quanfu Zheng & Chunyu Li

  3. Animal Laboratory Center, 302 Hospital of People’s Liberation Army, Bei**g, 100039, China

    Ruisheng Li

  4. Department of Integrative Medical Center, 302 Hospital of People’s Liberation Army, 100, the 4th Ring Road, Bei**g, 100039, People’s Republic of China

    Ruilin Wang, Honghong Liu & Jianyu Li

  5. Department of outpatient, 302 Hospital of People’s Liberation Army, 100, the 4th Ring Road, Bei**g, 100039, China

    Ye Wang

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  1. Quanfu Zheng
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Correspondence to Yanling Zhao.

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Quanfu Zheng, Ruisheng Li have contribute equally to this study/work and should be considered co-first authors.

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Zheng, Q., Li, R., Li, C. et al. Microcalorimetric investigation of five Aconitum L. plants on the metabolic activity of mitochondria isolated from rat liver. J Therm Anal Calorim 120, 335–344 (2015). https://doi.org/10.1007/s10973-014-4160-1

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