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Insights into the antioxidant and anticancer properties of novel biologically synthesized NiO/Ni2O3 nanoparticles using Sargassum tenerrimum

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Abstract

Presently, the utilization of nanomaterials has evolved as an appealing alternative for ever-changing healthcare obstacles due to their distinctive features and multifunctional applications. This work aimed to fabricate and analyze novel NiO/Ni2O3 nanoparticles (NPs) using Sargassum tentorium extracts and investigate their antioxidant and anticancer potentials. Diverse analytical instrumental tools were applied to explore NiO/Ni2O3 NPs, including UV–vis diffuse reflectance spectroscopy (UVDRS), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), high-resolution scanning electron microscopy (HRTEM), and energy-dispersive X-ray (EDX) analyses. HRTEM images revealed mostly quasi-spherical and wire-shaped NPs with average 97 and 87 nm sizes for NiO/Ni2O3 NPs. Afterward, the biological properties of the biologically manufactured NiO/Ni2O3 NPs were explored. This work assessed the anticancer potential of as-fabricated NiO/Ni2O3 NPs utilizing the MTT assay. The experiment assessed cell viability at doses ranging from 7.81 to 500 µg/mL during a 24 h period for the breast cancer cell line (MCF-7). The study outcomes demonstrate a concentration-dependent effect of fabricated NiO/Ni2O3 NPs on MCF-7 cells. The inhibition of MCF-7 cells increases with the concentration of NiO/Ni2O3 NPs, achieving an IC50 value of 883.4 µg/mL in 24 h. Furthermore, the antioxidant potency of NiO/Ni2O3 NPs was examined via a free radical scavenging ABTS and DPPH assay. At a concentration of 50 μg/mL, NiO/Ni2O3 NPs also exhibited 74.71% ABTS scavenging and 71.62% DPPH scavenging inhibition, respectively. In conclusion, NiO/Ni2O3 NPs manufactured via Sargassum tenerrimum extracts could be promising candidates for further biomedical applications.

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Highlights

  • First-time disclosed work on Sargassum tenerrimum-mediated creation of NiO/Ni2O3 NPs via a bio-inspired approach.

  • The manufactured NiO/Ni2O3 NPs were analyzed via UVDRS, FTIR, XRD, FESEM, HRTEM, and EDX analyses.

  • As-prepared NiO/Ni2O3 NPs was investigated for anticancer potential using MTT assay for MCF-7 cell lines.

  • Antioxidant potency of NiO/Ni2O3 NPs was revealed using ABTS and DPPH tests.

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Authors and Affiliations

  1. Department of Botany, Yashavantrao Chavan Institute of Science, Satara, 415001, Maharashtra, India

    Mukul Barwant & Vanita Karande

  2. Department of Chemistry, Sikkim Institute of Science & Technology, Sikkim University, Jorethang, Sikkim, India

    Parita Basnet

  3. Department of Chemistry, School of Chemical Engineering, Lovely Professional University, Phagwara, 144411, Punjab, India

    Deepak Kumar

  4. Department of Clinical Biochemistry, Zahedan University of Medical Sciences, Zahedan, Iran

    Saman Sargazi

  5. Cellular and Molecular Research Center, Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran

    Saman Sargazi

  6. Department of Natural and Mathematical Sciences, Faculty of Engineering, Tarsus University, Tarsus, Turkey

    Mahmoud Mirzaei

  7. Department of Applied Science, University of Technology, Baghdad, Iraq

    Majid S. Jabir

  8. Department of Chemistry, KPG Arts, Commerce & Science College, Savitribai Phule Pune University, Igatpuri, 422403, Maharashtra, India

    Dnyaneshwar Sanap

  9. Centre for Herbal Pharmacology & Environmental Sustainability, Chettinad Hospital and Research Institute, Chettinad Academy of Research & Education, Kelambakkam, 603103, Tamil Nadu, India

    Suresh Ghotekar

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  1. Mukul Barwant
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  2. Vanita Karande
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  4. Deepak Kumar
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  5. Saman Sargazi
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Contributions

Conceptualization (MB, SG), investigation (MB, DK), formal analysis (MB, SS, SG), validation (MM, MSJ), visualization (MB, DS), writing of original draft (MB, PB, SG), supervision (VK), reviewing & editing (VK, SG).

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Correspondence to Vanita Karande or Suresh Ghotekar.

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Barwant, M., Karande, V., Basnet, P. et al. Insights into the antioxidant and anticancer properties of novel biologically synthesized NiO/Ni2O3 nanoparticles using Sargassum tenerrimum. J Sol-Gel Sci Technol (2024). https://doi.org/10.1007/s10971-024-06446-1

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