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Protected activity of a phytase immobilized in mesoporous silica with benefits to plant phosphorus nutrition

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Abstract

Phosphorus (P) is one of the main nutrients required for plant growth and is absorbed and metabolized in the form of orthophosphate ions (Pi). In agriculture, P is a major component of fertilizers. Forecast shortages in P mine stocks within the coming decades call for alternative sources of this element for agricultural use. In the present study, we explore the possibility of incorporating phytase near the plant root system. Phytase is an enzyme capable of mobilizing the main organic form of P as phytate, which can represent up to 90 % of the total P stock in soils. We report the immobilization of phytase in KIT-6 silica mesoporous materials with large pores of 8.6 nm. The enzymatic activity is localized in the porous network, and the main kinetic features of the free phytase appear to be maintained within the functional material (pHopt = 5–5.5 and T opt = 55 °C). Most importantly, the enzyme immobilized in the pores shows a higher temperature stability and appears to be protected from protease degradation. Finally, phytase-loaded KIT-6 proved efficient at hydrolyzing phytate and delivering inorganic P to growing Medicago truncatula plants, which accumulated this newly available P in both roots and shoots.

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Acknowledgments

The authors thank Jean-Claude Yvin and Timac-Agro International for financial support (contract # 061021) of the collaborative project, the “Halle de Biotechnologies” (UMR IATE/UMR SPO, Montpellier, France) for the production of the recombinant phytase. Christophe Trouillefou is the recipient of a Ph.D. fellowship (CIFRE 98/2011) from TIMAC-Agro International and ANRT. We are grateful to Marie-France Driole for her help in the synthesis of the mesoporous materials, and to Francesco Di Renzo for fruitful discussions.

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Authors and Affiliations

  1. Institut Charles Gerhardt, UMR 5253, Equipe MACS, Ecole Nationale Supérieure de Chimie, Montpellier, France

    Christophe Marcel Trouillefou, Thomas Cacciaguerra, Frédérique Cunin & Emmanuel Belamie

  2. Montpellier SupAgro, UMR 1222, Eco&Sols, 2, Place Pierre Viala, 34060, Montpellier Cedex 02, France

    Christophe Marcel Trouillefou & Edith Le Cadre

  3. INRA, UMR 1222, Eco&Sols, 2, Place Viala, 34060, Montpellier Cedex 02, France

    Claude Plassard

  4. TIMAC-Agro International, Centre de Recherche en Agro-sciences, 55 Bd Jules Verger, 35800, Dinard, France

    Christophe Marcel Trouillefou

  5. Ecole Pratique des Hautes Etudes, Paris, France

    Emmanuel Belamie

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  1. Christophe Marcel Trouillefou
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Correspondence to Christophe Marcel Trouillefou or Emmanuel Belamie.

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Trouillefou, C.M., Le Cadre, E., Cacciaguerra, T. et al. Protected activity of a phytase immobilized in mesoporous silica with benefits to plant phosphorus nutrition. J Sol-Gel Sci Technol 74, 55–65 (2015). https://doi.org/10.1007/s10971-014-3577-0

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