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Evaluation of 99mTc-labeled glutathione as a colon cancer targeting probe

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Abstract

The present study was designed to unravel the bioevaluation of [99mTc]Tc-GSH in HT 29 colon cancer cell line as well as in experimentally induced colon cancer. The radiochemical yield was observed to be 95.93 ± 1.09%. In silico docking studies revealed that [99mTc]Tc-GSH gets well accommodated in the binding pocket of gamma-glutamyl transpeptidase in comparable orientation with lowest estimated binding energy − 81.90. Toxicity assays i.e. 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide and trypan blue exclusion assay showed that radiocomplex is non-toxic to HT 29 cells. In conclusion, in vitro and in vivo results indicated the [99mTc]Tc-GSH radiocomplex has tumor delineating features and also exhibits selectivity for colon tumor.

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Authors and Affiliations

  1. Centre for Nuclear Medicine (UIEAST), Panjab University, Chandigarh, 160014, India

    Sumeera Sidiq, Ravi Ranjan Kumar & Vijayta D. Chadha

  2. University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, India

    Neelima D. Passi

  3. Department of Biophysics, Panjab University, Chandigarh, India

    D. K. Dhawan

  4. Department of Nuclear Medicine, Post Graduate Institute for Medical Education and Research, Chandigarh, India

    Jaya Shukla & B. R. Mittal

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  1. Sumeera Sidiq
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Correspondence to Vijayta D. Chadha.

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Sidiq, S., Kumar, R.R., Passi, N.D. et al. Evaluation of 99mTc-labeled glutathione as a colon cancer targeting probe. J Radioanal Nucl Chem 327, 673–689 (2021). https://doi.org/10.1007/s10967-020-07563-1

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  • DOI: https://doi.org/10.1007/s10967-020-07563-1

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