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Enhancing Cell Migration on Polyetherimide-Grafted Fe3O4@SiO2-Labeled Umbilical Cord-Derived Mesenchymal Stem Cells Arrests in Intervertebral Disc Regeneration

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Abstract

Intervertebral disc regeneration (IDR) cell therapies aim to regenerate the disc's depleted IVD cells. As an outcome, encouraging the transplanted cells' migration towards the site of damage is critical. It was determined that umbilical cord mesenchymal stem cells (UC-MSCs) labelled with Fe3O4@SiO2 coated polyetherimide (PEI) nanomaterials (PEI/Fe3O4@SiO2 NMs) had a repair effect on degenerative disc disease in the rat caudal vertebral. To understand more about the impact of nanomaterials on UC-MSCs, we characterized UC-MSCs labelled with PEI/Fe3O4@SiO2 NMs and tested whether UC-MSCs could repair IDR in animals. We found UC-MSCs migration was aided by the expressions of CXCR4 in the PEI/Fe3O4@SiO2-labeled UC-MSCs group, which also had improved disc height and tissue performance structure and a more significant ratio of transplanted cells. This treatment also triggered significantly enhanced IVD regeneration, as supported by higher levels of ACAN expressions, type II collagen expression, and Sox-9 expression, as well as lower levels of Il-1β, Tnf-α, and Mmp-13 at both protein levels and mRNA than the unlabeled groups. We exhibited that systemic distribution of UC-MSCs labelled with the PEI/Fe3O4@SiO2 NMs could be a suitable procedure for speeding up and enhancing clinically useful UC-MSCs therapy for IDR.

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  1. Lijun Tian and Tonghao Wang have contributed equally to this work.

Authors and Affiliations

  1. Graduate School, Tian** Medical University, Tian**, 300070, China

    Lijun Tian, Tonghao Wang, Guowang Li, Yong** Li & Lilong Du

  2. The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, Inner Mongolia, China

    Lijun Tian

  3. The Second Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, Inner Mongolia, China

    Lingzhi Cui

  4. Department of Minimally Invasive Spine Surgery, Tian** Hospital, No.406, Jiefangnan Road, Hexi district, Tian**, 300211, China

    BaoShan Xu

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  1. Lijun Tian
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Tian, L., Wang, T., Li, G. et al. Enhancing Cell Migration on Polyetherimide-Grafted Fe3O4@SiO2-Labeled Umbilical Cord-Derived Mesenchymal Stem Cells Arrests in Intervertebral Disc Regeneration. J Clust Sci 34, 599–611 (2023). https://doi.org/10.1007/s10876-022-02238-1

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