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Surface-imprinted polymer coating l-cysteine-capped ZnS quantum dots for target protein specific recognition

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Abstract

This paper demonstrated a new method for preparing fluorescent molecularly imprinted polymer (MIP) for specific recognition of a target protein. The MIP-based fluorescent receptor was developed by coating MIP layer on the surface of l-cysteine modified Mn2+-doped ZnS quantum dots (QDs) using the surface molecular imprinting process. These MIP-QDs composites demonstrated fast adsorption kinetics, high stability, and good dispersibility in aqueous media. Since the fluorescence quenching of MIP-QDs composites is proportional to the concentration of the lysozyme, the MIP-based fluorescent receptor was successfully applied to the direct fluorescence quantification of lysozyme without further pretreatment. The MIP-based fluorescent receptor exhibited good selectivity and sensitivity for lysozyme detection. The optimum fluorescence intensity of the MIP-QDs was found to be at pH 6.0, and the linear range of lysozyme was from 0.1 to 2.0 μM with the detection limit of 25.2 nM. Moreover, the proposed fluorescent receptor was satisfactorily applied to the determination of lysozyme in real samples. This study provides a new approach for recognizing and detecting of specific proteins in biological samples.

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References

  1. Harrison JF, Lunt GS, Scott P, Blainey JD (1968) Urinary lysozyme, ribonuclease, and low-molecular-weight protein in renal disease. Lancet 1:371–375

    Article  Google Scholar 

  2. Cheng AKH, Ge B, Yu HZ (2007) Aptamer-based biosensors for label-free voltammetric detection of lysozyme. Anal Chem 79:5158–5164

    Article  Google Scholar 

  3. Levinson SS, Elin RJ, Yam L (2002) Light chain proteinuria and lysozymuria in a patient with acute monocytic leukemia. Clin Chem 48:1131–1132

    Google Scholar 

  4. Carstens C, Deckwart M, Webber-Witt M, Schäfer V, Eichhorn L, Brockow K, Fischer M, Christmann M, Paschke-Kratzin A (2014) Evaluation of the efficiency of enological procedures on lysozyme depletion in wine by an indirect ELISA method. J Agric Food Chem 62:6247–6253

    Article  Google Scholar 

  5. Viadl ML, Gautron J, Nys Y (2005) Development of an ELISA for quantifying lysozyme in hen egg white. J Agric Food Chem 53:2379–2385

    Article  Google Scholar 

  6. Cai Z, Chen G, Huang X, Ma M (2011) Determination of lysozyme at the nanogram level in chicken egg white using Resonance Rayleigh-scattering method with Cd-doped ZnSe quantum dots as probe. Sensor Actuat B Chem 157:368–373

    Article  Google Scholar 

  7. Lu X, Luo Z, Liu C, Zhao S (2008) Resonance Rayleigh scattering for detection of proteins in HPLC. J Sep Sci 31:2988–2993

    Article  Google Scholar 

  8. Sener G, Ozgur E, Yilmaz E, Uzun L, Say R, Denizli A (2010) Quartz crystal microbalance based nanosensor for lysozyme detection with lysozyme imprinted nanoparticles. Biosens Bioelectron 26:815–821

    Article  Google Scholar 

  9. Mihai I, Vezeanu A, Polonschii C, Albu C, Radu G-L, Vasilescu A (2015) Label-free detection of lysozyme in wines using an aptamer based biosensor and SPR detection. Sensors Actuat B Chem 206:198–204

    Article  Google Scholar 

  10. Chen YM, Yu CJ, Cheng TL, Tseng WL (2008) Colorimetric detection of lysozyme based on electrostatic interaction with human serum albumin-modified gold nanoparticles. Langmuir 24:3654–3660

    Article  Google Scholar 

  11. Liu S, Na W, Pang S, Shi F, Su X (2014) A label-free fluorescence detection strategy for lysozyme assay using CuInS(2) quantum dots. Analyst 139:3048–3054

    Article  Google Scholar 

  12. Li S, Gao Z, Shao N (2014) Non-covalent conjugation of CdTe QDs with lysozyme binding DNA for fluorescent sensing of lysozyme in complex biological sample. Talanta 129:86–92

    Article  Google Scholar 

  13. Kondekova M, Maier V, Ginterova P, Marak J, Sevcik J (2014) Analysis of lysozyme in cheese samples by on-line combination of capillary zone electrophoresis and mass spectrometry. Food Chem 153:398–440

    Article  Google Scholar 

  14. Liao YH, Brown MB, Martin GP (2001) Turbidimetric and HPLC assays for the determination of formulated lysozyme activity. J Pharmacy and Pharmacology 53:549–554

    Article  Google Scholar 

  15. Rodriguez MC, Rivas GA (2009) Label-free electrochemical aptasensor for the detection of lysozyme. Talanta 78:212–216

    Article  Google Scholar 

  16. Rohrbach F, Karadeniz H, Erdem A, Famulok M, Mayer G (2012) Label-free impedimetric aptasensor for lysozyme detection based on carbon nanotube-modified screen-printed electrodes. Anal Biochem 421:454–459

    Article  Google Scholar 

  17. Vasilescu A, Gaspar S, Mihai I, Tache A, Litescu SC (2013) Development of a label-free aptasensor for monitoring the self-association of lysozyme. Analyst 138:3530–3537

    Article  Google Scholar 

  18. Wang J, Liu B (2009) Fluorescence resonance energy transfer between an anionic conjugated polymer and a dye-labeled lysozyme aptamer for specific lysozyme detection. Chem Commun 17:2284–2286. doi:10.1039/B820001G

    Article  Google Scholar 

  19. Pellegrino L, Tirelli A (2000) A sensitive HPLC method to detect hen’s egg white lysozyme in milk and dairy products. Int Dairy J 10:435–442

    Article  Google Scholar 

  20. Kondeková M, Maierb V, Ginterováb P, Marák J (2014) Analysis of lysozyme in cheese samples by on-line combination of capillary zone electrophoresis and mass spectrometry. Food Chem 153:398–404

    Article  Google Scholar 

  21. Erdema A, Eksina E, Mutia M (2014) Chitosan–graphene oxide based aptasensor for the impedimetric detection of lysozyme. Colloid Surfaces B 115:205–211

    Article  Google Scholar 

  22. Haupt K (2003) Peer reviewed: molecularly imprinted polymers: the next generation. Anal Chem 75:376A–383A

    Article  Google Scholar 

  23. Gn Wulff (2002) Enzyme-like catalysis by molecularly imprinted polymers. Chem Rev 102:1–28

    Google Scholar 

  24. Haupt K, Mosbac K (2000) Molecularly imprinted polymers and their use in biomimetic sensors. Chem Rev 100:2495–2504

    Article  Google Scholar 

  25. Lin CI, Joseph AK, Chang CK, Lee YD (2004) Molecularly imprinted polymeric film on semiconductor nanoparticles: analyte detection by quantum dot photoluminescence. J Chromatogr A 1027:259–262

    Article  Google Scholar 

  26. Bossi A, Bonini F, Turner AP, Piletsky SA (2007) Molecularly imprinted polymers for the recognition of proteins: the state of the art. Biosens Bioelectron 22:1131–1137

    Article  Google Scholar 

  27. Janiak DS, Kofinas P (2007) Molecular imprinting of peptides and proteins in aqueous media. Anal Bioanal Chem 389:399–404

    Article  Google Scholar 

  28. Ge Y, Turner AP (2008) Too large to fit? Recent developments in macromolecular imprinting, Trends Biotechnol 26:218–224

    Google Scholar 

  29. Díaz-Díaz G, Antuña-Jiménez D, Carmen Blanco-López M, Jesús Lobo-Castañón M, Jand Miranda-Ordieres A, Tuñón-Blanco P (2012) New materials for analytical biomimetic assays based on affinity and catalytic receptors prepared by molecular imprinting. Trend Anal Chem 33:68–80

    Article  Google Scholar 

  30. Ding Z, Annie Bligh SW, Tao L et al (2015) Molecularly imprinted polymer based on MWCNT-QDs as fluorescent biomimetic sensor for specific recognition of target protein. Mat Sci Eng C 48:469–479

    Article  Google Scholar 

  31. Alivisatos AP (1996) Semiconductor clusters, nanocrystals, and quantum dots. Science 271:933

    Article  Google Scholar 

  32. Resch-Genger U, Grabolle M, Cavaliere-Jaricot S, Nitschke R, Nann T (2008) Quantum dots versus organic dyes as fluorescent labels. Nat Methods 5:763–775

    Article  Google Scholar 

  33. Algar WR, Massey M, Krull UJ (2009) The application of quantum dots, gold nanoparticles and molecular switches to optical nucleic-acid diagnostics. Trends Anal Chem 28:292–306

    Article  Google Scholar 

  34. Basabe-Desmonts L, Reinhoudt DN, Crego-Calama M (2007) Design of fluorescent materials for chemical sensing. Chem Soc Rev 36:993–1017

    Article  Google Scholar 

  35. Wei X, Zhou Z, Hao T, Li H, Yan Y (2015) Molecularly imprinted polymer nanospheres based on Mn-doped ZnS QDs via precipitation polymerization for room-temperature phosphorescence probing of 2,6-dichlorophenol. RSC Adv 5:19799–19806

    Article  Google Scholar 

  36. Zhang W, He XW, Li WY, Zhang YK (2012) Thermo-sensitive imprinted polymer coating CdTe quantum dots for target protein specific recognition. Chem Commun 48:1757–1759

    Article  Google Scholar 

  37. You CC, Miranda OR, Gider B, Ghosh PS, Kim I, Erdogan B, Krovi SA, Bunz UHF, Rotello VM (2007) Detection and identification of proteins using nanoparticle-fluorescent polymer ‘chemical nose’ sensors. Nat Nanotechnol 2:318–323

    Article  Google Scholar 

  38. Koneswaran M, Narayanaswamy R (2009) l-cysteine-capped ZnS quantum dots based fluorescence sensor for Cu2+ ion. Sensor Actuat B Chem 139:104–109

    Article  Google Scholar 

  39. Bian W, Wang F, Wei Y, Wang L, Liu Q, Dong W, Shuang S, Choi MMF (2015) Doped zinc sulfide quantum dots based phosphorescence turn-off/on probe for detecting histidine in biological fluid. Anal Chim Acta 856:82–89

    Article  Google Scholar 

  40. Tan L, Huang C, Peng R, Tang Y, Li W (2014) Development of hybrid organic-inorganic surface imprinted Mn-doped ZnS QDs and their application as a sensing material for target proteins. Biosens Bioelectron 61:506–511

    Article  Google Scholar 

  41. Wang HF, He Y, Ji TR, Yan XP (2009) Surface molecular imprinting on Mn-doped ZnS quantum dots for room-temperature phosphorescence optosensing of pentachlorophenol in water. Anal Chem 81:1615–1621

    Article  Google Scholar 

  42. Gattás-Asfura KM, Leblanc RM (2003) Peptide-coated CdS quantum dots for the optical detection of copper(ii) and silver(i). Chem Commun 21:2684–2685

    Article  Google Scholar 

  43. Tan L, Kang C, Xu S, Tang Y (2013) Selective room temperature phosphorescence sensing of target protein using Mn-doped ZnS QDs-embedded molecularly imprinted polymer. Biosens Bioelectron 48:216–223

    Article  Google Scholar 

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Authors and Affiliations

  1. School of Life, Bei**g Institute of Technology, No. 5 Zhongguancun South Street, Haidian District, Bei**g, 100081, People’s Republic of China

    **n Zhang, Shu Yang, Liquan Sun & Aiqin Luo

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  1. **n Zhang
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  2. Shu Yang
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  3. Liquan Sun
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  4. Aiqin Luo
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Correspondence to Aiqin Luo.

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Zhang, X., Yang, S., Sun, L. et al. Surface-imprinted polymer coating l-cysteine-capped ZnS quantum dots for target protein specific recognition. J Mater Sci 51, 6075–6085 (2016). https://doi.org/10.1007/s10853-016-9914-7

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  • DOI: https://doi.org/10.1007/s10853-016-9914-7

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