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Enantiomer separation of phenyllactic acid by HPLC with Hp-β-cyclodextrin as chiral mobile phase additive

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Abstract

In our current research work, a sensitive, specific, and economic HPLC method has been developed for the separation of phenyllactic acid (PLA) enantiomers. The effects of cyclodextrin (CD) type, column temperature, buffer pH, methanol content and CD concentration on enantioselective separation were investigated. Baseline chromatographic separation was achieved on an Inertsil ODS-SP (150 × 4.6 mm, i.d. 5 μm) column with HP-β-CD as chiral mobile phase additive. The LOD and LOQ for d-phenyllactic acid were 0.3 and 0.7 μg/mL, respectively. A good linear relationship was obtained in the concentration range of 0.71–5.13 μg/mL with r2 >0.999 for d-PLA. The percentage recovery of the d-PLA ranged from 94.1 to 103.2 in l-PLA. This method is simple and cost-saving, and could be easily applied for chiral discrimination and determination of the racemic PLA in an enantioselective study in quality control laboratory.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (Nos. 31071490 and 20976070) and the Fundamental Research Funds for the Central Universities (No. JUDCF10024).

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu AVE, Wuxi, 214122, People’s Republic of China

    Zhengyu **, Na Yang & Xueming Xu

  2. School of Food Science and Technology, Jiangnan University, 1800 Lihu AVE, Wuxi, 214122, People’s Republic of China

    ** Xu, Wenxiu Zhao, Yawei Ning, Mohanad Bashari, Yisheng Chen, Zhengyu **, Na Yang & Xueming Xu

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  1. ** Xu
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  2. Wenxiu Zhao
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  3. Yawei Ning
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  4. Mohanad Bashari
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  5. Yisheng Chen
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  6. Zhengyu **
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  7. Na Yang
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Correspondence to Xueming Xu.

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Xu, J., Zhao, W., Ning, Y. et al. Enantiomer separation of phenyllactic acid by HPLC with Hp-β-cyclodextrin as chiral mobile phase additive. J Incl Phenom Macrocycl Chem 76, 461–465 (2013). https://doi.org/10.1007/s10847-012-0228-3

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  • DOI: https://doi.org/10.1007/s10847-012-0228-3

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