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Short-term nutrient removal efficiency and photosynthetic performance of Ulva pseudorotundata (Chlorophyta): potential use for Integrated Multi-Trophic Aquaculture (IMTA)

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Abstract

Short-term N-NH4+ and N-NO3− removal by Ulva pseudorotundata under different initial nutrient concentrations and biomass stocking densities were investigated, monitoring in parallel photosynthetic parameters obtained by Pulse-Amplitude-Modulation (PAM) fluorometry of chlorophyll-a and evaluating biomass characteristics (CHN, proteins, carbohydrates). The results showed that U. pseudorotundata cultivated under solar radiation in a land-based outdoor pilot scale system was efficient in removing N-NH4+ and N-NO3− even in concentrations as high as 500 µmol L–1 ensuring complete water remediation in a period of 24 h and in all experimental conditions tested, however differences were observed depending on the stocking density and type of N-source. Treatments with N-NH4+ showed faster N removal than those with N-NO3−, whilst treatments with N-NO3− required more photosynthetic energy. Part of the ammonium removed in the respective treatments may have been volatilized as a combined effect of the pH increase related to photosynthesis and aeration in the tanks. The results presented here establish the fundamental parameters for the use of U. pseudorotundata as a biofiltering organism for effluents with high concentrations of N-NH4+ and N-NO3−.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We sincerely thank the Federal University of Santa Catarina (UFSC) and University of Malaga (UMA), especially the faculty members and technical staff of the Laboratory of Phycology (LAFIC—UFSC), Laboratory of Fotobiología y Biotecnología de Organismos acuáticos (FYBOA—UMA), research center Grice Hutchinson (UMA), and Servicios Centrales de Apoyo a la Investigación (SCAI—UMA) for providing space and resources for this work. We also thank Marta G. Sanchez, Fabian Lopez, David Paniagua, Roberto Abdala, Nathalie K. Peinado, and Ellie Bergstrom for their support and assistance in carrying out experiments, data collection and manuscript revision. Financial resources and scholarships were provided by: Andalusia Government (Spain) in the frame of the Projects (1) FACCO- UMA18-FEDER JA-162 and NAZCA-PY20-00458, Santa Catarina State Foundation for Research and Innovation Support (FAPESC process no 03/2017, Brazil), and Coordination for the Improvement of Higher Education Personnel (CAPES/PRINT process nos. 88887.470102/2019-00, Brazil). We also thanks the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for providing Post-Doctoral scholarship to E. Bastos (process no. 151637 / 2020-2) and financial support through research grants. This study is part of the Doctoral thesis of the first author to the UFSC Graduate Program in Biotechnology and Bioscience, Santa Catarina, Brazil.

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Authors and Affiliations

  1. Graduate Program in Biotechnology and Biosciences, Phycology Laboratory (LAFIC), Department of Botany, Center for Biological Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, Santa Catarina, 88040-900, Brazil

    Thaís Fávero Massocato, José Bonomi-Barufi & Leonardo Rubi Rörig

  2. Andalusian Institute of Blue Biotechnology and Development (IBYDA), Experimental Center Grice Hutchinson, Malaga University, Lomas de San Julián, 2, 29004, Malaga, Spain

    Victor Robles-Carnero, Julia Vega, Antonio Avilés & Félix López Figueroa

  3. Phycology Laboratory (LAFIC), Department of Botany, Center for Biological Sciences, Federal University of Santa Catarina (UFSC), Florianópolis, Santa Catarina, 88040-900, Brazil

    Eduardo Bastos

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Contributions

Massocato, T., Vega, J., Robles-Carnero, V., Avilés, A., Bonomi-Barufi, J., Rörig, L., and Figueroa, F. participated in the experimental design.

Massocato, T., Vega, J., and Robles-Carnero, V. participated in the experimental execution.

Massocato, T., Vega, J., Robles-Carnero, V., and Avilés, A. dedicated for the chemical analysis.

Massocato, T., Robles-Carnero, V., Bastos, E., Bonomi-Barufi, J., Rörig, L., and Figueroa, F. participated in the execution of statistical analysis and data interpretation.

Massocato, T., Bastos, E., Bonomi-Barufi, J., Rörig, L., and Figueroa, F. wrote, review and editing the manuscript text.

Bonomi-Barufi, J., Rörig, L., and Figueroa, F. supervised and provided funding acquisition.

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Correspondence to Thaís Fávero Massocato.

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Massocato, T.F., Robles-Carnero, V., Vega, J. et al. Short-term nutrient removal efficiency and photosynthetic performance of Ulva pseudorotundata (Chlorophyta): potential use for Integrated Multi-Trophic Aquaculture (IMTA). J Appl Phycol 35, 233–250 (2023). https://doi.org/10.1007/s10811-022-02888-8

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