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Identification and expression profiling of HSP20 genes in Neoporphyra haitanensis

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Abstract

Small heat-shock proteins (sHSPs/HSP20s) are closely related to algae and plant thermotolerance, but studies on HSP20 family in Neoporphyra haitanensis are scarce. In this study, a total of eight HSP20 members were identified from the genome of N. haitanensis. It was predicted that most of them encode acidic hydrophilic proteins, locating in various organelles as chloroplasts and endoplasmic reticulum. Phylogenetically, the HSP20 genes of N. haitanensis were closely related to that of other species of Porphyra sensu lato, which evolved independently from that of land plants. In addition, their promoter regions contain a large number of cis-acting elements related to stress response. Based on the expression analysis of six HSP20 genes, it showed that the expression patterns of these genes were different among three conditions of dehydration, heat stress and co-stresses of heat and dehydration, with some genes being very sensitive to heat stress. These results initially indicated that HSP20 genes in N. haitanensis could specifically response to heat stress under gene expression level, suggesting their involvement in the process of thermotolerance of N. haitanensis, which is valuable for further studies on the molecular function of HSP20s in N. haitanensis.

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Data availability

The datasets generated during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors want to thanks Dr. Dong-Mei Wang (Ocean University of China) for the help on genomic data analysis.

Funding

This work was supported by the National Key Research and Development Program of China [2018YFD0900606]; the Startup Foundation for Young Teachers of Shanghai Ocean University; and the Open Program of Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province [2020fjscq01].

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Authors and Affiliations

  1. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China

    Ling-Na Peng, Lin-Bin Huang, Tian-Yu Gui, Da-Hai Gao & **ng-Hong Yan

  2. Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, 201306, China

    Ling-Na Peng, Lin-Bin Huang, Tian-Yu Gui, Da-Hai Gao & **ng-Hong Yan

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  1. Ling-Na Peng
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Correspondence to **ng-Hong Yan.

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Peng, LN., Huang, LB., Gui, TY. et al. Identification and expression profiling of HSP20 genes in Neoporphyra haitanensis. J Appl Phycol 34, 1089–1097 (2022). https://doi.org/10.1007/s10811-022-02686-2

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