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Increased activity of the tandem fructose-1,6-bisphosphate aldolase, triosephosphate isomerase and fructose-1,6-bisphosphatase enzymes in Anabaena sp. strain PCC 7120 stimulates photosynthetic yield

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Abstract

The regulation of photosynthetic yield at the genetic level has largely focused on manipulation of the catalytic enzymes in the Calvin cycle by genetic engineering. In order to investigate the contribution of increased enzymatic activity in the Calvin cycle on photosynthetic yield, the rice fructose-1,6-bisphosphate aldolase (FBA), spinach triosephosphate isomerase (TPI) and wheat fructose-1,6-bisphosphatase (FBPase) genes were cloned in tandem and co-overexpressed in cyanobacterium Anabaena sp. strain PCC 7120 cells. The enzymatic activities of FBA, TPI and FBPase, as well as sedoheptulose-1,7-bisphosphatase (SBPase), were remarkably increased in transgenic cells relative to the wild-type. The photosynthetic yield, as reflected by photosynthetic O2 evolution and dry cellular weight, was also markedly increased in transgenic cells versus wide-type cells. The activity of SBPase is considered the most important factor for ribulose-1,5-bisphosphate (RuBP) regeneration in the Calvin cycle, and increased activity of TPI alone in transgenic cells does not stimulate photosynthetic yield. Thus, the increased activity of FBA and FBPase, but not TPI, significantly improved photosynthetic yield in transgenic cells by stimulating SBPase activity and consequently accelerating the RuBP regeneration rate.

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Abbreviations

CS:

control strain harboring pDC-08

DHAP:

dihydroxyacetone phosphate

FBA:

fructose-1,6-bisphosphate aldolase

FBP:

fructose-1,6-bisphosphate

F-6-P:

fructose-6-bisphosphate

FBPase:

fructose-1,6-bisphosphatase

G-3-P:

D-glyceraldehyde-3-phosphate

RuBP:

ribulose-1,5-bisphosphate

SBPase:

sedoheptulose-1,7-bisphosphatase

TPI:

triosephosphate isomerase

TS:

transgenic strain harboring pDC-FTF

WT:

wild-type

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Acknowledgements

The authors are deeply grateful to Prof. Tang GL (Institute of Organic Chemistry, Chinese Academy of Sciences) for providing the pDCFAT plasmid and fruitful discussion. The authors also thank Prof. Wolk CP (Michigan State University) for providing the pDC-08, RP4 and pRL623 plasmids. This work was partially supported by the National Natural Science Foundation of China (No. 30770175) and the Key Fundamental Project of Shanghai (06JC14091).

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Authors and Affiliations

  1. College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, People’s Republic of China

    Weimin Ma, Lanzhen Wei, Quanxi Wang & Dingji Shi

  2. Laboratory of Photosynthesis, Institute of Botany, Chinese Academy of Sciences, Bei**g, 100093, People’s Republic of China

    Weimin Ma & Dingji Shi

  3. State Key Laboratory of Bio-organic and Natural Products Chemistry, Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, 200032, People’s Republic of China

    Haibao Chen

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  1. Weimin Ma
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  2. Lanzhen Wei
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  4. Dingji Shi
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Correspondence to Weimin Ma or Quanxi Wang.

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Ma, W., Wei, L., Wang, Q. et al. Increased activity of the tandem fructose-1,6-bisphosphate aldolase, triosephosphate isomerase and fructose-1,6-bisphosphatase enzymes in Anabaena sp. strain PCC 7120 stimulates photosynthetic yield. J Appl Phycol 20, 437–443 (2008). https://doi.org/10.1007/s10811-007-9286-0

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  • DOI: https://doi.org/10.1007/s10811-007-9286-0

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