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The Effect of Porphyromonas gingivalis Lipopolysaccharide on the Pyroptosis of Gingival Fibroblasts

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Abstract

Periodontitis is a chronic inflammatory disease induced by Porphyromonas gingivalis (P. gingivalis) and other pathogens. P. gingivalis release various virulence factors including lipopolysaccharide (LPS). However, whether P. gingivalis–LPS inducing pyroptosis in human gingival fibroblasts (HGFs) remains unknown. In present study, P. gingivalis–LPS decreased the membrane integrity of HGFs, and pyroptosis-associated cytokines were upregulated at the mRNA level. In addition, pyroptosis proteins were highly expressed in gingival tissues of periodontitis. P. gingivalis–LPS induced gingivitis in the rat model, and the expression level of pyroptosis-associated proteins increased. Together, P. gingivalis–LPS can activate the pyroptosis reaction, which may be a pro-pyroptosis status in a relative low concentration.

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Funding

The study was supported by the grant from the Finance Department of Jilin Province (JCSZ2019378-3; JCSZ2020304-24) and the grants from the Department of Science and Technology of Jilin Province (20180101121JC; 20200404108YY).

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Authors and Affiliations

  1. Department of Dental Implantology, Hospital of Stomatology, Jilin University, Changchun, Jilin, China

    Yu-Yang Li, Qing Cai, Bao-Sheng Li, Shu-Wei Qiao, Jia-Yang Jiang, Dan Wang, Xue-Chun Du & Wei-Yan Meng

  2. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun, Jilin, China

    Yu-Yang Li, Shu-Wei Qiao, Jia-Yang Jiang, Dan Wang & Xue-Chun Du

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  1. Yu-Yang Li
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Li, YY., Cai, Q., Li, BS. et al. The Effect of Porphyromonas gingivalis Lipopolysaccharide on the Pyroptosis of Gingival Fibroblasts. Inflammation 44, 846–858 (2021). https://doi.org/10.1007/s10753-020-01379-7

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