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The Effect of Synthetic Salidroside on Cytokines and Airway Inflammation of Asthma Induced by Diisocyanate (TDI) in Mice by Regulating GATA3/T-bet

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Abstract

This study was conducted to explore the anti-asthma effects of synthetic salidroside on cytokines and airway inflammation of asthma induced by diisocyanate (TDI) in mice. The experiment was carried out 60 female BALB/C mice which were randomly assigned to six experimental groups: control, vehicle, model, dexamethasone (2 mg/kg), and salidroside (24 and 48 mg/kg). After the experiment, histological studies were evaluated by the hematoxylin and eosin staining, the bronchoalveolar lavage fluid (BALF) and blood were collected from the animals, and the composition of the induced inflammatory cells, and the concentrations of certain cytokines (IL-4, INF-γ) were evaluated. GATA3 and T-bet mRNAs were evaluated by QPCR. Our study demonstrated that salidroside inhibited TDI-induced increases in eosinophil count; IL-4 and INF-γ were recovered. Histological studies demonstrated that salidroside substantially inhibited TDI-induced eosinophilia in lung tissue. Salidroside can improve T-bet mRNA and reduce GATA3 mRNA in lung. These findings suggest that salidroside may effectively ameliorate the progression of asthma and could be used as a therapy for patients with allergic asthma by regulating GATA3/T-bet balance.

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Acknowledgments

This work was supported by National twelve five major drug discovery project (2011ZX09102-002-01).

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Authors and Affiliations

  1. Department of Physiology and Pharmacology, China Pharmaceutical University, Tongjiaxiang 24, Nan**g, China

    **g Wang, Lu **ao, Lingpeng Zhu, Mei Hu, Qiujuan Wang & Tianhua Yan

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  1. **g Wang
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  2. Lu **ao
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  3. Lingpeng Zhu
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  4. Mei Hu
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  5. Qiujuan Wang
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  6. Tianhua Yan
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Correspondence to Tianhua Yan.

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Wang, J., **ao, L., Zhu, L. et al. The Effect of Synthetic Salidroside on Cytokines and Airway Inflammation of Asthma Induced by Diisocyanate (TDI) in Mice by Regulating GATA3/T-bet. Inflammation 38, 697–704 (2015). https://doi.org/10.1007/s10753-014-9979-x

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