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GhLIP1, a lipoic acid synthase gene, negatively regulates leaf senescence in cotton

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Abstract

Leaves are the key organs of plants that produce photosynthates. Leaf senescence aids nutrient remobilization from source to sink tissues, which dramatically affects crop quality and yield. Although several senescence-associated genes (SAGs) have been recognized in cotton, the function of lipoic acid synthase in leaf senescence remains unclear. Therefore, we isolated a lipoic acid synthase gene named GhLIP1 from cotton (Gossypium hirsutum L.). Protein domain analysis revealed that GhLIP1 had a LIAS-N domain and an Elp3 domain, and yeast mutant complementation experiments demonstrated that GhLIP1 was a lipoic acid synthase. GhLIP1 expression pattern analysis showed that GhLIP1 was vastly expressed in fibres, and the phytohormone IAA (indole-3-acetic acid) induced its transcription. When GhLIP1 was transformed into Arabidopsis, the leaves of overexpression Arabidopsis exhibited a late leaf senescence phenotype compared to wild-type Arabidopsis, Col-0. Moreover, downregulation of GhLIP1 expression using virus-induced gene silencing (VIGS) technology resulted in early leaf senescence in cotton. qRT-PCR analyses revealed that two SAGs, GhWRKY53 and GhNAP, were upregulated in cotton plants with GhLIP1 knock-down. Cell ultrastructure observations found that chloroplasts were degraded in an orderly manner in cotton leaf cells with GhLIP1 knock-down. These results suggest that GhLIP1 plays a significant role in leaf senescence in cotton.

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Acknowledgements

We thank Dr. Shijun Chen of the Experimental Center at Henan Institute of Science and Technology for his help with the cell ultrastructure observations.

Funding

This work was supported by the National Natural Science Foundation of China (Grant Number 31872129) and Henan Science and Technology Project (Grant Number 202102110146).

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  1. Eryong Chen and Haiyan Hu have contributed equally to this work.

Authors and Affiliations

  1. Henan Collaborative Innovation Center of Modern Biological Breeding, School of Life Science and Technology, Henan Institute of Science and Technology, **nxiang, 453003, Henan, China

    Eryong Chen, Haiyan Hu, **aobei Yang, Dongxiao Li, Qichao Wei, Feng Zhou, Yuanyuan Guan, Yongang Yu, Puwen Song & Chengwei Li

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  1. Eryong Chen
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  2. Haiyan Hu
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Contributions

EY Chen and CW Li conceived and designed the research; EY Chen, HY Hu, XB Yang, DX Li and QC Wei performed the experiments; F Zhou, YY Guan, YA Yu and PW Song analyzed the data; EY Chen and CW Li wrote, reviewed and edited the manuscript. All authors read and approved the final manuscript.

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Correspondence to Chengwei Li.

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Fig. S1

Multiple sequence alignment of the GhLIP1 protein and its homologs in other species. (PDF 16 kb)

Fig. S2

Phenotypes of cotton plants with down-regulated expression of GhLIP1 and GhPDS using VIGS technology. The TRV2::GhPDS cotton line was used as a positive control in the VIGS procedure. (TIF 7179 kb)

Table S1

Primers used in this study. Table S2 The qRT-PCR primers specifically used for the analysis of GhLIP1 and senescence-associated gene expression patterns. (DOCX 15 kb)

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Chen, E., Hu, H., Yang, X. et al. GhLIP1, a lipoic acid synthase gene, negatively regulates leaf senescence in cotton. Plant Growth Regul 94, 73–85 (2021). https://doi.org/10.1007/s10725-021-00697-6

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