Abstract
The genetic diversity of fig (Ficus carica L.) germplasm in Azerbaijan was assessed using pomological traits and inter-simple sequence repeat (ISSR) markers. All 90 accessions exhibited significant variation in fruit-related traits. The studied fig accessions displayed a full spectrum of fruit skin ground colors, from yellow to black, with yellow–green (32.2%) and yellow (26.6%) being the most prevalent colors. The majority of the fruits were categorized as globose (73%). Fruit weight among the fig accessions varied from 18.8 g (‘Nikitskiy 4067’) to 83 g (‘Konservniy’), with an average of 44 g. Azerbaijani figs demonstrated similar weight ranges, with 13 local accessions exceeding 50 g, including ‘Iri Absheron’, ‘Agh Enjir’, and ‘Vuqarli’. Two-way cluster analysis based on these traits identified fruit weight and dimensions as the primary grou** factors. Preliminary ISSR analysis using eight primers revealed polymorphism levels ranging from 40 to 100%, with an average of 63.5%. The mean genetic diversity among the 90 fig genotypes was calculated to be 0.65. Local Azerbaijani accessions exhibited comparable diversity to those from Ukraine and lower diversity than those from the United States. The Jaccard genetic distance index among the accessions was low. Both Azerbaijani and introduced accessions in the neighbor-joining (NJ) tree showed a random distribution that was independent of origin, which was further confirmed by principal coordinate analysis (PCoA). These results provide a valuable baseline for future breeding programs and can aid in the identification and conservation of these genetic resources.
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Data availability
All data generated during this study are included in this published article and its supplementary information files.
References
Abbasi K, Arji I (2014) Pomological diversity of fig (Ficus carica L.) accessions of Kermanshah. Iran J Bio Env Sci 5(3):202–209
Abdelsalam NR, Awad RM, Ali HM, Salem MZ, Abdellatif KF, Elshikh MS (2019) Morphological, pomological, and specific molecular marker resources for genetic diversity analyses in fig (Ficus carica L.). Hort Sci 54(8):1299–1309
Ahmad FK, Noori IM (2023) Evaluation of genetic diversity of figs (Ficus carica L.) in Sulaymaniyah governorate using morphological, pomological and specific molecular markers. Tikrit J Agric Sci 23(4):147–175
Ahmadov ACİ (2020) Mükəmməl meyvə-Əncir [excellent fruit-fig]. Elm, Bakı
Ahmed BA, Ghada B, Laila E, Hafid A, Bouchaib K, Amel SH (2015) Use of morphological traits and microsatellite markers to characterize the Tunisian cultivated and wild figs (Ficus carica L.). Biochem Syst Ecol 59:209–219. https://doi.org/10.1016/j.bse.2015.01.026
Aljane F, Nahdi S, Essid A (2012) Genetic diversity of some accessions of Tunisian fig tree (Ficus carica L.) based in morphological and chemical traits. J Nat Prod Plant Resour 2(3):350–359
Aljane F, Essid A, Nahdi S (2018) Improvement of fig (Ficus carica L.) by conventional breeding and biotechnology. In: Al-Khayri JM, Jain SM, Johnson DV (eds) Advances in plant breeding strategies: fruits. Springer, Cham, pp 343–375
Almajali DA, Abdel-Ghani AH, Migdadi H (2012) Evaluation of genetic diversity among Jordanian fig germplasm accessions by morphological traits and ISSR markers. Sci Hortic 147:8–19
Aradhya MK, Stover E, Velasco D, Koehmstedt A (2010) Genetic structure and differentiation in cultivated fig (Ficus carica L.). Genetica 138:681–694
Babayeva S, Akparov Z, Amirov L, Shikhaliyeva K, Hasanova S, Rustamov K, Abbasov M (2018) Genetic relationship among introduced lentil germplasm using agronomic traits and ISSR markers. Genetika 50(2):575–590
Baraket G, Chatti K, Saddoud O, Mars M, Marrakchi M, Trifi M, Salhi-Hannachi A (2009) Genetic analysis of Tunisian fig (Ficus carica L.) cultivars using amplified fragment length polymorphism (AFLP) markers. Sci Hortic 120:487–492
Boudchicha RH, Hormaza JI, Benbouza H (2018) Diversity analysis and genetic relationships among local Algerian fig cultivars (Ficus carica L.) using SSR markers. South Afr J Bot 116:207–215
Çalişkan O, Polat AA (2008) Fruit characteristics of fig cultivars and genotypes grown in Turkey. Sci Hortic 115(4):360–367
Çalişkan O, Polat AA (2012) Morphological diversity among fig (Ficus carica L accessions sampled from the Eastern Mediterranean Region of Turkey. Turk J Agric For 36(2):179–193
Chatti K, Salhi-Hannachi A, Mars M, Marrakchi M, Trifi M (2004) Analyse de la diversité génétique de cultivars tunisiens de figuier (Ficus carica L.) à l’aide de caractères morphologiques. Fruits 59(1):49–61
Chinapolaiah A, Bindu KH, Khadke GN, Manjesh GN, Rao NH, Kumar SS, Suthar MK (2018) Genetic diversity analysis in underutilized medicinal climber Mucuna pruriens L. DC. germplasm revealed by inter simple sequence repeats markers. Legume Res 43:32–37. https://doi.org/10.18805/LR-3954
Condit IJ (1955) Fig varieties: a monograph. Hilgardia 23(11):323–538
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Essid A, Aljane F, Neily MH, Ferchichi A, Hormaza JI (2021) Assessment of genetic diversity of thirty Tunisian fig (Ficus carica L.) accessions using pomological traits and SSR markers. Mol Biol Rep 48:335–346
FAO (Food and Agricultural Organization) (2022) www.fao.org
Gaaliche B, Lauri PE, Trad M, Costes E, Mars M (2011) Interactions between vegetative and generative growth and between crop generations in fig tree (Ficus carica L.). Sci Hortic 131:22–28
Ganopoulos I, Kalivas A, Kavroulakis N, Xanthopoulou A, Mastrogianni A, Koubouris G, Madesis P (2015) Genetic diversity of Barbary fig (Opuntia ficus-indica) collection in Greece with ISSR molecular markers. Plant Gene 2:29–33
Giraldo E, Lopez-Corrales M, Hormaza JI (2008) Optimization of the management of an ex-situ germplasm bank in common fig with SSRs. J Am Soc Hortic Sci 133(1):69–77
Hammer O, Harper DAT, Ryan PD (2001) Palaeontol Electron 4(1):1–9
Hasanov NA, Hasanova AH (2016) Əncir genofondunun toplanması və öyrənilməsi [Collection and study of fig gene pool]. Proc Res Inst Crop Husb 26:103–109
Hssaini L, Hanine H, Razouk R, Ennahli S, Mekaoui A, Ejjilani A, Charafi J (2020) Assessment of genetic diversity in Moroccan fig (Ficus carica L.) collection by combining morphological and physicochemical descriptors. Genet Resour Crop Evol 67(2):457–474. https://doi.org/10.1007/s10722-019-00838-x
Ikegami H, Nogata H, Hirashima K, Awamura M, Nakahara T (2009) Analysis of genetic diversity among European and Asian fig varieties (Ficus carica L.) using ISSR, RAPD, and SSR markers. Genet Resour Crop Evol 56(2):201–209. https://doi.org/10.1007/s10722-008-9355-5
Ikten H, Mutlu N, Gulsen O, Kocatas H, Aksoy U (2010) Elucidating genetic relationships, diversity and population structure among the Turkish female figs. Genetica 138:169–177
IPGRI and CIHEAM (2003) Descriptors for fig (Ficus carica L.). International Plant Genetic Resources Institute (IPGRRI), Paris
Karaat FE (2022) Morphological characterization of promising fig (Ficus carica L.) genotypes from Southeastern Turkey. Erwerbs-Obstbau 64(3):1–10. https://doi.org/10.1007/s10341-022-00651-4
Khadivi-Khub A, Anjam K (2014) Characterization and evaluation of male fig (caprifig) accessions in Iran. Plant Syst Evol 300:2177–2189
Kjellberg F, Gouyon PH, Ibrahim M, Raymond M, Valdeyron G (1987) The stability of the symbiosis between dioecious figs and their pollinators: a study of Ficus carica L. and Blastophaga psenes L. Evolution 41(4):693–704
Mammadov TS (2010) Abşeronun ağac və kolları [Trees and shrubs of Absheron]. Elm, Bakı
Mint Abdelaziz S, Medraoui L, Alami M, Pakhrou O, Makkaoui M et al (2020) Inter simple sequence repeat markers to assess genetic diversity of the desert date (Balanites aegyptiaca Del.) for Sahelian ecosystem restoration. Sci Rep 10(1):14948
Mubaslat AK (2012) Genetic diversity among Palestinian fig varieties (Ficus carica L.) using ISSR, and RAPD markers. Dissertation, An-Najah National University
Oukabli A, Mamouni A, Laghezali R, Khadari B, Roger JP, Kjellberg F, Ater M (2002) Genetic variability in Morrocan fig cultivars (Ficus carica) based on morphological and pomological data. Acta Hortic 605:54–60
Patil VV, Patil VR (2011) Evaluation of anti-inflammatory activity of Ficus carica Linn. leaves. Indian J Nat Prod Res 2(2):151–155
Pereira C, Lopez-Corrales M, Serradilla MJ, Villalobos MDC, Ruiz-Moyano S, Martin A (2017) Influence of ripening stage on bioactive compounds and antioxidant activity in nine fig (Ficus carica L.) varieties grown in Extremadura. Spain J Food Compos Anal 64:203–212
Perez-Jiménez M, López B, Dorado G, Pujadas-Salvá A, Guzmán G, Hernandez P (2012) Analysis of genetic diversity of southern Spain fig tree (Ficus carica L.) and reference materials as a tool for breeding and conservation. Hereditas 149(3):108–113
Perrier X, Jacquemoud-Collet JP (2006) DARwin software http://darwin.cirad.fr/darwin
Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed 2:225–238
Prevost A, Wilkinson MJ (1999) A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor Appl Genet 98:107–111
Rodolfi M, Silvanini A, Chiancone B, Marieschi M, Fabbri A, Bruni R, Ganino T (2018) Identification and genetic structure of wild Italian Humulus lupulus L. and comparison with European and American hop cultivars using nuclear microsatellite markers. Genet Resour Crop Evol 65:1405–1422
Roldan-Ruiz I, Dendauw J, Bockstaele VE, Depicker A, De Loose MAFLP (2000) AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Mol Breed 6(2):125–134
Saddoud O, Salhi-Hannachi A, Chatti K, Mars M, Rhouma A, Marrakchi M, Trifi M (2005) Tunisian fig (Ficus carica L.) genetic diversity and cultivar characterization using microsatellite markers. Fruits 60(2):143–153
Sclavounos A, Roussos P, Milla S, Kostas P, Samaras Y, Pozzi C, Voloudakis A (2023) Genetic diversity of fig (Ficus carica L.) germplasm from the Mediterranean basin as revealed by SSR markers. Genet Resour Crop Evol 70(5):1395–1406
SPSS Inc. Released 2007. SPSS for Windows, Version 16.0. Chicago, SPSS Inc
Stover E, Aradhya M, Ferguson L, Crisosto CH (2007) The fig:overview of an ancient fruit. Hortic Sci 42(5):1083–1087
The state Statistical Committee of the Republic of Azerbaijan (2022). https://www.stat.gov.az/source/agriculture/
Treder W (2008) Relationship between yield, crop density coefficient and average fruit weight of ‘Gala’ apple. J Fruit Ornam Plant Res 16:53–63
Vavilov NI (1951) The origin, variation, immunity and breeding of cultivated plants. LWW, Philadelphia
Weir BS (1990) Genetic-data analysis methods for discrete genetic data. Sinauer Assoc Inc, Sunderland
Zhang X, Kong W, Wang X, Zhang J, Liu L, Wang W, Deng Q (2020) Genetic diversity analysis of 34 fig varieties (Ficus carica L.) based on ISSR molecular marker. Genet Resour Crop Evol 67:913–921
Zohary D, Hopf M (2000) Domestication of plants in the Old World: the origin and spread of cultivated plants in West Asia Europe and the Nile Valley. Oxford University Press, Oxford
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Mehraj Abbasov played a pivotal role in formulating the original problem and offering invaluable direction and guidance throughout the study. Qamar Qurbanova led the field and molecular analysis. Sevda Babayeva and Qamar Qurbanova conducted thorough data analysis and collaborated closely to write the manuscript. All authors read and approved the final manuscript.
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Qurbanova, Q., Babayeva, S. & Abbasov, M. Analysis of the genetic diversity of Azerbaijani fig accessions (Ficus carica L.) using pomological traits and inter simple sequence repeat (ISSR) markers. Genet Resour Crop Evol (2024). https://doi.org/10.1007/s10722-024-02072-6
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DOI: https://doi.org/10.1007/s10722-024-02072-6