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Effects of fasting, temperature, and photoperiod on preproghrelin mRNA expression in Chinese perch

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Abstract

Preproghrelin, a gut/brain peptide, plays an important role in the regulation of food intake and energy homeostasis in teleost and mammals. In the present study, we obtained the full-length preproghrelin cDNA in Chinese perch. The preproghrelin messenger RNA (mRNA) tissue expression showed that level was much higher in stomach and pituitary than in other tissues. The fasting study showed, after gastric emptying (3–6 h), short-term fasting (6–12 h) increased preproghrelin expression in the stomach. While in the pituitary, fasting reduced preproghrelin expression at 1, 3, 12, and 48 h, presenting state fluctuation of self-adjustment. The temperature study showed that the mRNA expression of preproghrelin was the highest in the brain at 26 °C and highest in the stomach at 32 °C, respectively, with different optimum temperature in these two tissues, reflecting spatiotemporal differences of regulation by central nervous system and peripheral organs. The photoperiod study showed that normal light (11 h of lightness and 13 h of darkness) led to highest preproghrelin expression, both in the brain and in the stomach, than continuous light or continuous dark, proving food intake is adapted to natural photoperiod or normal light in this study. These results all indicated that tissue-specific preproghrelin expression of Chinese perch could be significantly affected by environmental factors. Short-term fasting of 6 h after gastric emptying, 26 °C, and normal light led to higher preproghrelin expression, which indicated potential appetite increase in Chinese perch.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (31272641), the Key Projects in the National Science & Technology Pillar Program (2012BAD25B04), and the Fundamental Research Funds for the Central Universities (2662015PY041 and 2015BQ040).

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Authors and Affiliations

  1. College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, People’s Republic of China

    Yi Song, Cheng Zhao, Xu-fang Liang, Shan He, Changxu Tian, **aoyan Cheng, **aochen Yuan, Liyuan Lv & Wenjie Guo

  2. Freshwater Aquaculture Collaborative Innovation 8 Center of Hubei Province, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China

    Yi Song, Cheng Zhao, Xu-fang Liang, Shan He, Changxu Tian, **aoyan Cheng, **aochen Yuan, Liyuan Lv & Wenjie Guo

  3. National Aquafeed Safety Assessment Station, Feed Research Institute, the Chinese Academy of Agricultural Sciences, Bei**g, People’s Republic of China

    Min Xue

  4. Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, AL, 36849, USA

    Ya-**ong Tao

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  1. Yi Song
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Correspondence to Xu-fang Liang.

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Song, Y., Zhao, C., Liang, Xf. et al. Effects of fasting, temperature, and photoperiod on preproghrelin mRNA expression in Chinese perch. Fish Physiol Biochem 43, 803–812 (2017). https://doi.org/10.1007/s10695-016-0335-y

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