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Insulin-like growth factor binding protein-2 (IGFBP-2) in Japanese flounder, Paralichthys olivaceus: molecular cloning, expression patterns and hormonal regulation during metamorphosis

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Abstract

In this study, we cloned and characterized cDNA sequences of two insulin-like growth factor binding protein-2 (IGFBP-2a and IGFBP-2b) from Japanese flounder, Paralichthys olivaceus. The full-length cDNA of IGFBP-2a is 1,046 bp long and consists an open frame (ORF) of 876 bp, a 5′-untranslated region (UTR) of 125 bp and a 3′-UTR of 45 bp. IGFBP-2b is 1,067 bp, including a 5′-UTR of 53 bp, a 3′-UTR of 198 bp and an ORF of 816 bp. Real-time quantitative PCR results revealed that IGFBP-2a -2b mRNA was expressed in all detected tissues. Interestingly, the levels of IGFBP-2a mRNA in all detected tissues were higher in female than male, but IGFBP-2b was precisely the opposite. At different embryonic stages, the levels of IGFBP-2a mRNA were typically higher than IGFBP-2b. After hatching, IGFBP-2a mRNA was gradually decreased to a relatively lower level. However, the expression of IGFBP-2b mRNA was increased after hatching, including 3, 7, 10, 14, 17, 20 and 23 days post-hatching (dph), and it presents a higher level until 29 (metamorphic climax), 36 (post-climax) and 41 dph (the end of metamorphosis). In levothyroxine sodium salt (T4, the main form of thyroid hormone in animals)-treated and thiourea (TU)-treated larvae, the expressions of IGFBP-2a had not visibly changed, except in T4-treated 17 dph larvae. The expressions of IGFBP-2b mRNA were distinctly increased from 17 to 23 dph, but suddenly dropped to a lower level in and after 29 dph. However, the levels of IGFBP-2b mRNA during metamorphosis were greatly down-regulated after TU treatment. These results provided basic information for further studies on the role of IGF system in flatfish development and metamorphosis.

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Acknowledgments

The authors wish to thank Prof. Hai** Liu (Chinese Academy of Fishery Sciences) for providing embryos and larvae samples and all the staff in Beidaihe Central Experimental Station for their helps in the raising of experimental fish. This research was supported by the National Natural Science Foundation of China (No. 31172392), Leading Academic Discipline Project of Shanghai Municipal Education Commission (No. S30701; No. J50701) and Dr. Foundation of Shanghai Ocean University.

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  1. Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, People’s Republic of China

    Yuntong Zhang, Junling Zhang, Zhiyi Shi, Wanying Zhai & **aozhu Wang

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  1. Yuntong Zhang
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  2. Junling Zhang
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Correspondence to Zhiyi Shi.

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Yuntong Zhang and Junling Zhang have contributed equally to this work.

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Zhang, Y., Zhang, J., Shi, Z. et al. Insulin-like growth factor binding protein-2 (IGFBP-2) in Japanese flounder, Paralichthys olivaceus: molecular cloning, expression patterns and hormonal regulation during metamorphosis. Fish Physiol Biochem 39, 1541–1554 (2013). https://doi.org/10.1007/s10695-013-9807-5

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