Abstract
Recovery from caudal artery cannulation with and without pre-anaesthesia metomidate sedation was assessed in Atlantic cod (Gadus morhua). The levels of plasma cortisol, glucose, electrolytes and acid–base parameters were compared between sedated and unsedated cod and to those in uncannulated individuals, where the samples were obtained by sacrificial sampling (reference level). Metomidate sedation delayed the stress response, causing sedated cod plasma cortisol to return to the reference level more slowly [day 4 post surgery (PS)] than in unsedated cod (day 2 PS). Plasma glucose was elevated in both sedated and unsedated cod up to and including day 5 PS. Plasma K+ was lower and pH was higher in cannulated cod than in the reference from 24 h PS until the end of experimentation, indicating a stress effect of sacrificial sampling on plasma K+ and pH that was likely caused by an acute stress response. Metomidate sedation delayed the stress response following CA cannulation and should therefore not be used as a pre-anaesthetic sedation in Atlantic cod. The caudal artery cannulation can be a useful tool in obtaining repeated blood samples from Atlantic cod given an adequate recovery time, which was determined to be 6 days irrespective of pre-anaesthesia sedation status.
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Acknowledgments
This study was funded by the Norwegian Research Council (NFR) through the research projects PROCOD (NFR project number 172263) and MODSMO (NFR project number 172514). We would like to thank the staff at NIVA-MFS for their technical assistance and care for the fish prior to and during the experiment, and an anonymous reviewer for the critical comments and suggestions on how to improve the manuscript.
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Karlsson, A., Rosseland, B.O., Massabuau, JC. et al. Pre-anaesthetic metomidate sedation delays the stress response after caudal artery cannulation in Atlantic cod (Gadus morhua). Fish Physiol Biochem 38, 401–411 (2012). https://doi.org/10.1007/s10695-011-9516-x
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DOI: https://doi.org/10.1007/s10695-011-9516-x