Abstract
Purpose
Acid-tolerant barley has a one-kbp insertion at the 5’ untranslated region of HvAACT1. Marker-assisted breeding based on the one-kbp at the 5’ UTR enhances acid tolerance in barley. Under acidic growing conditions, a two-year experiment was conducted to determine the variability and interrelationships of 12 quantitative traits that regulate yield in the F2 and F3 populations of the cross between E232 and Murasakimochi and their resprocal cross. A higher phenotypic coefficient of variation indicated a prevalence of adequate variability for the measured traits. The kernels per spike and plant ranged from 11–95 seeds per spike and 0–668, respectively. Plant height and shoot length ranged from 51 to 155 cm and 2.5 to 17 cm, respectively. Thousand seed weights and yield per plant ranged from 0–73 g to 0–36 g/plant, respectively. The peduncle length, peduncle extrusion length, flag leaf width, and flag leaf length showed similar patterns of variation: 14 to 51 cm, 3.4 to 30.7 cm, 4.1\(-\)70.5 cm, and 0.3\(-\)0.6 cm, respectively. A positive correlation was observed for all traits. There was positive skewness with the number of tillers, yield per plant, kernels per plant, and thousand seed weights. Kernel per spike, shoot length, flag leaf width, and plant height showed negative skewness, which indicates duplicate epistasis of dominant genes in trait inheritance. The biplot analysis showed that the mean performance of all measured traits was high between genotypes with the one-kbp insertion of the HvAACT1 gene. The lines without insertion have the lowest mean value.Marker-assisted selection accelerates the selection of barley lines with acid tolerance one-kbp insertion.
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The corresponding author will provide the data used and examined during the current study upon reasonable request.
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The Ohara-Foundation and International Joint Research Program, Institute of Plant Science and Resources at Okayama University, which provided funding for the study, are gratefully acknowledged by the authors.
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This research is funded by Ohara Foundation and International Joint Research Program, Institute of Plant Science and Resources at Okayama University
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KS came up with the research design, crossing the parental lines. HDD and AM were involved in the fieldwork. The data collection and data entry were performed by AM. The corresponding author, HDD, performed the manuscript’s data analysis and writing, while the other coauthors read and revised it. The final manuscript was read and approved by all authors.
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Degu, H.D., Semahegn, A. & Sato, K. Marker-assisted selection of one-kbp insertion at the 5’ UTR region of HvAACT1 gene improves plant performance under acidic soil growth conditions in barley (Hordeum vulgare). Euphytica 219, 122 (2023). https://doi.org/10.1007/s10681-023-03241-x
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DOI: https://doi.org/10.1007/s10681-023-03241-x