Log in

Determination of melamine contamination in milk with various packaging: a risk assessment study

  • Research
  • Published:
Environmental Monitoring and Assessment Aims and scope Submit manuscript

Abstract

Melamine is one type of monomer used as starting substance in manufacturing packaging lining in many countries worldwide. Environmental and food contamination is an issue constantly discussed. In the present study, the melamine content in milk samples with three package types was measured by HPLC/UV. Melamine is not a lipophilic compound. Therefore, the selected samples were low-fat milk. The melamine content in various packaged milk, including packet, polyethylene bags, and plastic packaging, is 790 ± 39.8, 50.7 ± 13, and 57.7 ± 24.54 ppb, respectively. According to the existing standards, the measured values in all the milk samples were lower than the permitted limits. The risk assessment for adults and children showed that the HQ value for both age groups was less than 1. Therefore, milk consumption will not pose a health risk in terms of contamination with melamine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Germany)

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

Availability of data and material

The datasets generated and analyzed during the current study available from the corresponding author on reasonable request.

References

  • Abedini, R., Jahed Khaniki, G., Molaee Aghaee, E., Sadighara, P., Nazmara, S., Akbari-Adergani, B., & Naderi, M. (2021). Determination of melamine contamination in chocolates containing powdered milk by high-performance liquid chromatography (HPLC). Journal of Environmental Health Science and Engineering, 19, 165–171.

    Article  CAS  Google Scholar 

  • Aleksic, B., Djekic, I., Miocinovic, J., Miloradovic, Z., Memisi, N., & Smigic, N. (2022). The application of failure mode effects analysis in the long supply chain–A case study of ultra filtrated milk cheese. Food Control, 138, 109057.

    Article  Google Scholar 

  • Alimentarius, C. (1995). Codex general standard for contaminants and toxins in foods. Codex Stan, 193, 30–33.

    Google Scholar 

  • Bischoff, K. (2022). Chapter 26 - Melamine and cyanuric acid. In R. C. Gupta (Ed.), Reproductive and Developmental Toxicology (Third Edition) (pp. 503–511). Academic Press.

    Chapter  Google Scholar 

  • Bouma, K., Kalsbeek-van Wijk, D. K., & Sijm, D. T. H. M. (2022). Migration of formaldehyde from ‘biobased’ bamboo/melamine cups: A Dutch retail survey. Chemosphere, 292, 133439.

    Article  CAS  Google Scholar 

  • Caldara, M., Lowdon, J. W., Royakkers, J., Peeters, M., Cleij, T. J., Diliën, H., Eersels, K., & van Grinsven, B. (2022). A molecularly imprinted polymer-based thermal sensor for the selective detection of melamine in milk samples. Foods, 11, 2906.

    Article  CAS  Google Scholar 

  • dos-Santos, L. & Suguimoto, H.H. (2014). Challenges about determining the amount of melamine in dairy products. Int J Dairy Sci Process, 2, 1–2.

    Google Scholar 

  • Filazi, A., Sireli, U., Ekici, H., Can, H., & Karagoz, A. (2012). Determination of melamine in milk and dairy products by high performance liquid chromatography. Journal of Dairy Science, 95, 602–608.

    Article  CAS  Google Scholar 

  • Fischer, W., Schilter, B., Tritscher, A., & Stadler, R. (2011). Contaminants of milk and dairy products: Contamination resulting from farm and dairy practices. Encyclopedia of Dairy Sciences, 2, 887–897.

    Article  Google Scholar 

  • García Londoño, V. A., Puñales, M., Reynoso, M., & Resnik, S. (2018). Melamine contamination in milk powder in Uruguay. Food Additives & Contaminants. Part b, Surveillance, 11, 15–19.

    Article  Google Scholar 

  • Haghi, E., Alimohammadi, M., Shakoori, A., Razeghi, F., & Sadighara, P. (2018). Measurement of melamine migration from melamine-ware products by designed HPLC method and the effect of food-type on the level of migration. Interdisciplinary Toxicology, 11, 316.

    Article  CAS  Google Scholar 

  • Jie, L., **g, X., Da-**, Y., Zhu-Tian, W., Jiang, D.-G., Cong-Rong, F., & Jie, Y. (2009). Study on migration of melamine from food packaging materials on markets. Biomedical and Environmental Sciences, 22, 104–108.

    Article  Google Scholar 

  • **gbin, W., Ndong, M., Kai, H., Matsuno, K., & Kayama, F. (2010). Placental transfer of melamine and its effects on rat dams and fetuses. Food and Chemical Toxicology, 48, 1791–1795.

    Article  CAS  Google Scholar 

  • Karlsson, M.A., Langton, M., Innings, F., Malmgren, B., Höjer, A., Wikström, M. & Lundh, Å. (2019) Changes in stability and shelf-life of ultra-high temperature treated milk during long term storage at different temperatures. Heliyon, 5.

  • Levinson, L. R., & Gilbride, K. A. (2011). Detection of melamine and cyanuric acid in vegetable protein products used in food production. Journal of Food Science, 76, C568–C575.

    Article  CAS  Google Scholar 

  • Li, J., Gao, X., He, Y., Wang, L., Wang, Y., & Zeng, L. (2022). Elevated emissions of melamine and its derivatives in the indoor environments of typical e-waste recycling facilities and adjacent communities and implications for human exposure. Journal of Hazardous Materials, 432, 128652.

    Article  CAS  Google Scholar 

  • Liu, C. C., Hsieh, T. J., Wu, C. F., Lee, C. H., Tsai, Y. C., Huang, T. Y., Wen, S. C., Lee, C. H., Chien, T. M., Lee, Y. C., Huang, S. P., Li, C. C., Chou, Y. H., Wu, W. J., & Wu, M. T. (2020). Interrelationship of environmental melamine exposure, biomarkers of oxidative stress and early kidney injury. Journal of Hazardous Materials, 396, 122726.

    Article  CAS  Google Scholar 

  • Petrus, R. R., Loiola, C., & Oliveira, C.A.F.d. (2010). Microbiological shelf life of pasteurized milk in bottle and pouch. Journal of Food Science, 75, M36–M40.

    Article  CAS  Google Scholar 

  • Rajpoot, M., Bhattacharya, R., Sharma, S., Gupta, S., Sharma, V., & Sharma, A. K. (2021). Melamine contamination and associated health risks: Gut microbiota does make a difference. Biotechnology and Applied Biochemistry, 68, 1271–1280.

    CAS  Google Scholar 

  • Sadighara, P., Mofid, V., Mahmudiono, T., Rahmani, A., Tajdar-Oranj, B., Peivasteh-Roudsari, L., Farhangfar, A., Moradi, M. & Fakhri, Y. (2022) Concentration of heavy metals in canned tuna fish and probabilistic health risk assessment in Iran. International Journal of Environmental Analytical Chemistry, 1–11.

  • Shishov, A., Nizov, E., & Bulatov, A. (2023). Microextraction of melamine from dairy products by thymol-nonanoic acid deep eutectic solvent for high-performance liquid chromatography-ultraviolet determination. Journal of Food Composition and Analysis, 116, 105083.

    Article  CAS  Google Scholar 

  • Singh, P., & Gandhi, N. (2015). Milk preservatives and adulterants: Processing, regulatory and safety issues. Food Reviews International, 31, 236–261.

    Article  CAS  Google Scholar 

  • Sun, H., Wang, L., Ai, L., Liang, S., & Wu, H. (2010). A sensitive and validated method for determination of melamine residue in liquid milk by reversed phase high-performance liquid chromatography with solid-phase extraction. Food Control, 21, 686–691.

    Article  CAS  Google Scholar 

  • Tian, Y., Chen, L., Gao, L., Michel, F. C., Keener, H. M., Klingman, M., & Dick, W. A. (2012). Composting of waste paint sludge containing melamine resin and the compost’s effect on vegetable growth and soil water quality. Journal of Hazardous Materials, 243, 28–36.

    Article  CAS  Google Scholar 

  • Tittlemier, S. A., Lau, B.P.-Y., Ménard, C., Corrigan, C., Sparling, M., Gaertner, D., Cao, X.-L., & Dabeka, B. (2010). Baseline levels of melamine in food items sold in Canada. I. Dairy products and soy-based dairy replacement products. Food Additives and Contaminants, 3, 135–139.

    Article  CAS  Google Scholar 

  • Tiwari, M., Singh, A., Dureja, S., Basu, S., & Pattanayek, S. K. (2022). Au nanoparticles decorated ZnO/ZnFe2O4 composite SERS-active substrate for melamine detection. Talanta, 236, 122819.

    Article  CAS  Google Scholar 

  • Tyan, Y.-C., Yang, M.-H., Jong, S.-B., Wang, C.-K., & Shiea, J. (2009). Melamine contamination. Analytical and Bioanalytical Chemistry, 395, 729–735.

    Article  CAS  Google Scholar 

  • Xu, L., Liu, H., Chua, T. C., Hong, M., & Zhou, H. (2022). Fabrication of SERS substrates by femtosecond LIPAA for detection of contaminants in foods. Optics & Laser Technology, 151, 107954.

    Article  CAS  Google Scholar 

  • Xu, X.-M., Ren, Y.-P., Zhu, Y., Cai, Z.-X., Han, J.-L., Huang, B.-F., & Zhu, Y. (2009). Direct determination of melamine in dairy products by gas chromatography/mass spectrometry with coupled column separation. Analytica Chimica Acta, 650, 39–43.

    Article  CAS  Google Scholar 

  • Yan, L., Wu, M., Zhang, Z., Zhou, Y., Lin, L., Fang, E., Xu, D., & Chen, L. (2008). Determination of melamine residue in raw milk and dairy products using hydrophilic interaction chromatography-electrospray ionization tandem mass spectrometry. Se Pu, 26, 759–762.

    CAS  Google Scholar 

Download references

Acknowledgements

We thank Shahid Beheshti University of Medical Sciences.

Funding

This study received financial support from Shahid Beheshti University of Medical Sciences (project No: 22003).

Author information

Authors and Affiliations

Authors

Contributions

The analysis of melamine in samples was conducted by Hadi Eghbaljoo, Nader Akbari, Babak Mahmoodi, and Parisa Sadighara. The analysis of data was performed by Saeed Aghebat-Bekheir, Kiandokht Ghanati, and Yeganeh Mazaheri. The manuscript was written by Kiandokht Ghanati and Yeganeh Mazaheri, Farshid Zandsalimi, and Burhan Basaran.

Corresponding author

Correspondence to Parisa Sadighara.

Ethics declarations

Ethical approval

Not applicable. All authors have read, understood, and complied as applicable with the statement on “Ethical responsibilities of Authors” as found in the Instructions for Authors.

Consent to participate

Informed consent was obtained from all individual participants included in the study.

Consent for publication

Not applicable.

Competing interests

The authors declare no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ghanati, K., Eghbaljoo, H., Akbari, N. et al. Determination of melamine contamination in milk with various packaging: a risk assessment study. Environ Monit Assess 195, 1095 (2023). https://doi.org/10.1007/s10661-023-11737-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s10661-023-11737-1

Keywords

Navigation