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Modulation of Rat Intestinal Nuclear Factor NF-κB by Gum Arabic

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Abstract

The objective of this study was to test the hypothesis that in an animal model of cathartic-induce intestinal dysfunction the proabsorptive effects of gum arabic (GA) could be associated with modulation of nuclear factor-κB (NF-κB) and with reduction of the inflammatory response caused by cathartics, as evidenced by intestinal mucosa cytokine production and gene expression. Juvenile male rats were given a phenolphthalein–magnesium citrate solution for 6 days, by itself or supplemented with either 10 or 20 g L−1 GA, as a sole source of fluid. The controls given were tap water alone or with added 20 g L−1 GA. The animals were euthanized and small-intestinal mucosa nuclear fractions and RNA were isolated. NF-κB p65 activity was highest after administration of cathartics, lowest in controls, and intermediate in GA-treated rats. Mucosal IL-1β was overexpressed in tissues from cathartic-treated rats and from rats given high-GA solutions. Gene-array analysis revealed a complex pattern of gene regulation by cathartics which selectively upregulated several subfamilies of cytochrome P-450 family 2 genes. Co-administration of GA did not block this effect. These findings suggest that local anti-inflammatory effects on the small intestine could be obtained by administration of a nonabsorbable proteoglycan such as GA.

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Acknowledgements

We thank Dr Alamelu Chandrasekaran and Sarah Lombardi for their excellent assistance in conducting QRT-PCR and microarray gene chip analysis.

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Authors and Affiliations

  1. The Feinstein Institute for Medical Research, North Shore-Long Island Jewish Health System, 350 Community Drive, Manhasset, NY, 11030, USA

    Raul A. Wapnir, Barbara Sherry & Leslie O. Goodwin

  2. Division of Neonatal/Perinatal Medicine, Schneider Children’s Hospital at North Shore, 350 Community Drive, Manhasset, NY, 11030, USA

    Champa N. Codipilly

  3. Department of Biology, St. John’s University, Queens, NY, 11439, USA

    Ivana Vancurova

Authors
  1. Raul A. Wapnir
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  2. Barbara Sherry
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  3. Champa N. Codipilly
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  4. Leslie O. Goodwin
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  5. Ivana Vancurova
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Correspondence to Raul A. Wapnir.

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Supported in part by a grant of The Feinstein Institute for Medical Research

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Wapnir, R.A., Sherry, B., Codipilly, C.N. et al. Modulation of Rat Intestinal Nuclear Factor NF-κB by Gum Arabic. Dig Dis Sci 53, 80–87 (2008). https://doi.org/10.1007/s10620-007-9826-0

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  • DOI: https://doi.org/10.1007/s10620-007-9826-0

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