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Effect of abiotic stresses on the activity of antioxidative enzymes and contents of phytohormones in wild type and AtCKX2 transgenic tobacco plants

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Biologia Plantarum

Abstract

The responses of antioxidant enzymes (AOE) ascorbate peroxidase (APX), glutathione reductase (GR), superoxide dismutase (SOD), and catalase (CAT) in soluble protein extracts from leaves and roots of tobacco (Nicotiana tabacum L. cv. Samsun NN) plants to the drought stress, salinity and enhanced zinc concentration were investigated. The studied tobacco included wild-type (WT) and transgenic plants (AtCKX2) harbouring the cytokinin oxidase/dehydrogenase gene under control of 35S promoter from Arabidopsis thaliana (AtCKX2). The transgenic plants exhibited highly enhanced CKX activity and decreased contents of cytokinins and abscisic acid in both leaves and roots, altered phenotype, retarded growth, and postponed senescence onset. Under control conditions, the AtCKX2 plants exhibited noticeably higher activity of GR in leaves and APX and SOD in roots. CAT activity in leaves always decreased upon stresses in WT while increased in AtCKX2 plants. On the contrary, the SOD activity was enhanced in WT but declined in AtCKX2 leaves. In roots, the APX activity prevailingly increased in WT while mainly decreased in AtCKX2 in response to the stresses. Both WT and AtCKX2 leaves as well as roots exhibited elevated abscisic acid content and increased CKX activity under all stresses while endogenous CKs and IAA contents were not much affected by stress treatments in either WT or transgenic plants.

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Abbreviations

ABA:

abscisic acid

AtCKX2:

transgenic tobacco harbouring cytokinin oxidase/dehydrogenase gene from Arabidopsis thaliana

AOE:

antioxidative enzymes

APX:

ascorbate peroxidase

β-car:

β-carotene

CAT:

catalase

Chl:

chlorophyll

CK:

cytokinin

CKX:

cytokinin oxidase/dehydrogenase

DEPS:

deepoxidation state

GR:

glutathione reductase

SOD:

superoxide dismutase

WT:

wild type

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Acknowledgement

This work was financially supported by the Grant Agency of the Czech Republic, projects No.522/03/0312 and 206/06/1306. The authors wish to thank Ing. P. Dobrev, Ing. J. Malbeck and Ing. A. Trávníčková (Institute of Experimental Botany AS CR, Prague, Czech Republic) for HPLC separation and MS analyses of phytohormones and M. Korecká for excellent technical assistance. Z.M. and V.M. contributed equally to this work.

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Authors and Affiliations

  1. Faculty of Science, Charles University, Albertov 6, Praha 2, CZ-12843, Czech Republic

    Z. Mýtinová, D. Haisel & Z. Lubovská

  2. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Karlovce 1a, CZ-16000, Prague, Czech Republic

    Z. Mýtinová, D. Haisel, Z. Lubovská & N. Wilhelmová

  3. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, CZ-16502, Prague, Czech Republic

    V. Motyka & A. Gaudinová

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  1. Z. Mýtinová
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  2. V. Motyka
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  3. D. Haisel
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  4. A. Gaudinová
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  5. Z. Lubovská
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  6. N. Wilhelmová
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Correspondence to N. Wilhelmová.

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Mýtinová, Z., Motyka, V., Haisel, D. et al. Effect of abiotic stresses on the activity of antioxidative enzymes and contents of phytohormones in wild type and AtCKX2 transgenic tobacco plants. Biol Plant 54, 461–470 (2010). https://doi.org/10.1007/s10535-010-0082-3

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  • DOI: https://doi.org/10.1007/s10535-010-0082-3

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