Abstract
Patients with chronic pelvic inflammation (CPI) experience irregular menstrual, ectopic pregnancy, and infertility. Yiyi Baijiang Decoction attenuates CPI in patients with uncovered mechanisms. CPI therapeutic targets intersected with those of Yiyi Baijiang Decoction, followed by importing into STRING to obtain protein–target interaction. “Drug-component-disease-target” interaction was constructed by Cytoscape. mRNA and protein levels were detected by real-time quantitative PCR (RT-qPCR) and western blot. Yiyi Baijiang Decoction contained 199 active ingredients. There were 1071 drug targets for Yiyi Baijiang Decoction and 1622 therapeutic targets for CPI. The GO functional enrichment analysis revealed 3445 biological processes, and the KEGG pathway enrichment analysis screened 67 signal pathways. Decreased ALB, increased protein kinase B (AKT1), interleukin (IL)-6, vascular endothelial growth factor A (VEGFA), and phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K/AKT)-extracellular-regulated protein kinases (ERK)1/2 activation in CPI mice were abolished by Yiyi Baijiang Decoction. Yiyi Baijiang Decoction attenuates CPI by inactivating PI3K/AKT and ERK1/2 and regulating ALB, VEGFA, AKT1, and IL-6.
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The data used to support the findings of this study are available from the corresponding author upon request.
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The present study is funded by the Scientific Research Project of Hebei Provincial Administration of Traditional Chinese Medicine (2020282).
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YW, HS, YS, and MZ conceived and designed the experiments. YW, HS, JL, WZ, and LL carried out the experiments, analyzed the data, and drafted the manuscript. YW, HS, JL, and YS participated in its design and prepared the paper. YW, HS, YS, and MZ revised the manuscript. All authors read and approved the final manuscript.
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Ethical approval for this study (SY2020-01) was provided by the Experimental Animal Ethics Committee of Hebei Ex&Invivo Biotechnology Co., Ltd on 10 February 2020.
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Wei, Y., Su, H., Li, J. et al. Exploration into the Mechanism of Yiyi Baijiang Decoction Attenuating Chronic Pelvic Inflammation Based on Network Pharmacology and Experimental Verification. Biochem Genet (2024). https://doi.org/10.1007/s10528-024-10843-0
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DOI: https://doi.org/10.1007/s10528-024-10843-0