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Effects of Wenyang Zhenshuai Granules on the Expression of Key Mitochondrial Autophagy Proteins in the Doxorubicin-Induced Model of H9c2 Cardiomyocyte Injury

  • PHARMACOLOGY AND TOXICOLOGY
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Bulletin of Experimental Biology and Medicine Aims and scope

This study aimed to explore the effects of Wenyang Zhenshuai granules (WZG) on the morphology of cardiomyocytes, cell viability, and the expression of key mitochondrial autophagy proteins in the doxorubicin-induced model of H9c2 cardiomyocyte injury. Cardiomyocytes were cultured for 44 h and divided into 4 groups: intact control, doxorubicin-injured cells (DOX), doxorubicin-injured cells treated with WZG (DOX+WZG), and doxorubicin-injured cells treated with valsartan (DOX+valsartan; reference group). The morphology of cardiomyocytes was analyzed under an inverted microscope; cardiomyocyte survival rate was determined by MTT assay. The expression of the key mitochondrial autophagy proteins (PINK1, parkin, LC3-II, and prohibitin-2) was analyzed by Western blotting. WZG down-regulated the expression of the key mitochondrial autophagy proteins in DOX-injured cells, which may be one of the important mechanisms for regulating ventricular remodeling and cardiomyocyte apoptosis.

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Authors and Affiliations

  1. The First Affiliated Hospital of Hunan University of Chinese Medicine, Hunan, China

    L. Q. Peng, H. Z. Cai, Q. Y. Chen & X. Y. Chen

  2. Chengdu **ndu District Traditional Chinese Medicine Hospital, Sichuan, China

    X. X. Wu

  3. Liuyang Hospital of Traditional Chinese Medicine, Hunan, China

    G. Chen

  4. College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Hunan, China

    Y. P. Tang

Authors
  1. L. Q. Peng
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  2. X. X. Wu
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  3. G. Chen
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  4. H. Z. Cai
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  5. Y. P. Tang
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  6. Q. Y. Chen
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  7. X. Y. Chen
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Corresponding authors

Correspondence to Q. Y. Chen or X. Y. Chen.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 173, No. 3, pp. 328-334, March, 2022

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Peng, L.Q., Wu, X.X., Chen, G. et al. Effects of Wenyang Zhenshuai Granules on the Expression of Key Mitochondrial Autophagy Proteins in the Doxorubicin-Induced Model of H9c2 Cardiomyocyte Injury. Bull Exp Biol Med 173, 335–340 (2022). https://doi.org/10.1007/s10517-022-05545-9

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  • DOI: https://doi.org/10.1007/s10517-022-05545-9

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