Abstract
A Gram-stain-negative, rod-shaped, polar flagellated or stalked and non-spore-forming bacterium, designated LB-2T, was isolated from activated sludge. Growth was observed at 20–30 °C (optimum 28 °C), pH 6.0–8.0 (optimum pH 7.0) and salinity of 0–0.5% (w/v; optimum 0.5%). Phylogenetic analysis based on the 16S rRNA gene indicated that strain LB-2T belongs to the genus Sphingomonas and showed the highest sequence similarity (96.7%) and less than 96.7% similarities to other type strains. The genome size of strain LB-2T was 4.10 Mb, with 66.8 mol% G + C content. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strains LB-2T and S. canadensis FWC47T were 77.8% and 21%, respectively. The predominant cellular fatty acids were summed feature 8 (C18:1ω7c and/or C18 : 1ω6c) and C16:0. The major polar lipids were aminolipid, glycolipid, sphingoglycolipid, phosphatidylcholine, phosphatidylglycerol, four unidentified lipids, glycophospholipid, phosphatidylethanolamine and diphosphatidylglycerol. The predominant respiratory quinone was Q-10 and the major polyamine was sym-homospermidine. On the basis of phenotypic, genotypic and phylogenetic evidences, strain LB-2T represents a novel species in the genus Sphingomonas, for which the name Sphingomonas caeni sp. nov. is proposed. The type strain is LB-2T (GDMCC 1.3630T = NBRC 115,102T).
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Data availability
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain LB-2T is OP610062. The whole-genome shotgun sequence of strains LB-2T and S. canadensis FWC47T have been deposited in GenBank/EMBL/DDBJ under the accession numbers JAPDOK000000000 and JAPDRA000000000, respectively.
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Funding
This work was supported by the Natural Science Foundation of Jiangxi Province (20224BAB215005, 20202BAB213024), the Special fund for science and technology program of Jiangsu province (BM2022019) and the Youth Technology Fund Project of Gansu Province (20JR5RA124).
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Conceptualization, funding acquisition, and supervision: HN; Laboratory work, data analysis and writing-original draft: BL; Writing-review and editing: YW, EC, MH, XC, JH. All authors read and approved the final manuscript.
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Liu, B., Wan, Y., Chen, E. et al. Sphingomonas caeni sp. nov., a phenolic acid-degrading bacterium isolated from activated sludge. Antonie van Leeuwenhoek 116, 687–695 (2023). https://doi.org/10.1007/s10482-023-01837-w
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DOI: https://doi.org/10.1007/s10482-023-01837-w