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Characterization of a strong CCA-treated wood degrader, unknown Crustoderma species

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Abstract

In this study, basidiomycete isolates that possessed a strong ability to degrade chromated copper arsenate (CCA)-treated wood were characterized. These fungal isolates, which were collected from CCA-treated pine log wastes, showed no recognizable morphological properties on culture media. Nucleotide sequence analysis of the large subunit rDNA of the isolates revealed that they were one species. Based on the high sequence similarity (>95%) and close phylogenetic relationship with several known species of Crustoderma, the fungal isolates characterized in this study were classified as a Crustoderma sp. In a wood degradation test, Crustoderma isolate KUC8611 produced a remarkably higher weight loss in CCA-treated Pinus radiata (68.7%), Pseudotsuga menziesii (39.7%), and Tsuga heterophylla (38.5%) wood than other evaluated basidiomycete species, including Crustoderma flavescens and Crustoderma corneum. In addition, extracellular enzymes for cellulose and protein degradation were detected when the isolates were cultured in chromogenic media, which supports the finding that isolate KUC8611 is a wood degrader. Furthermore, an in vitro test for metal tolerance revealed that isolate KUC8611 showed strong arsenic tolerance, but that it could not tolerate copper. Finally, isolate KUC8611 produced lower amounts of oxalic acid than copper-tolerant fungi such as Fomitopsis palustris and Antrodia vaillantii. To the best of our knowledge, this is the first study to report the degradation of CCA-treated wood by a Crustoderma species.

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Acknowledgments

This work was supported by the KTOL grant funded by The National Instituted of Biological Resources, Korean Government.

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Authors and Affiliations

  1. Division of Environmental Science & Ecological Engineering, College of Life Sciences & Biotechnology, Korea University, 5-1 Anam-dong, Seongbuk-Gu, Seoul, 136-701, Korea

    Yong-Seok Choi, Gyu-Hyeok Kim & Jae-** Kim

  2. National Institute of Biological Resources (NIBR), Incheon, 404-708, Korea

    Young Woon Lim

  3. Department of Microbiology and Institute of Basic Sciences, Dankook University, Cheonan, Chungnam, 330-714, Korea

    Seong Hwan Kim

  4. Laboratory of Innovative Humano-habitability, Research Institute for Sustainable Humanosphere (RISH), Kyoto University, Uji, Kyoto, 611-0011, Japan

    Yuji Imamura & Tsuyoshi Yoshimura

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  1. Yong-Seok Choi
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  7. Jae-** Kim
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Correspondence to Jae-** Kim.

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Choi, YS., Kim, GH., Lim, Y.W. et al. Characterization of a strong CCA-treated wood degrader, unknown Crustoderma species. Antonie van Leeuwenhoek 95, 285–293 (2009). https://doi.org/10.1007/s10482-009-9311-1

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  • DOI: https://doi.org/10.1007/s10482-009-9311-1

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