Abstract
The goal of this study was to evaluate the efficacy of the chimeric marker vaccine candidate CP7_E2alf in different formulations for oral immunization against classical swine fever (CSF). In the first experiment, three wild boars were vaccinated orally with the liquid chimeric virus CP7_E2alf, whereas in the second experiment, four wild boars and four domestic pigs were immunized with the lyophilized chimera administered in gelatine capsules and new baits. The chimera was safe for wild boars and domestic pigs. All animals vaccinated orally with the liquid chimera were seropositive 21 days after vaccination, and neither viraemia nor virus shedding were observed after challenge with a highly virulent CSF virus. At necropsy, viral genome of the challenge virus was detected in some organs of all surviving wild boars. Lyophilized chimera administered orally did not protect the animals. The reasons for the inefficacy of the lyophilized vaccine virus are discussed. Irrespective of the negative result with lyophilized virus, the study and previous experiences (Reimann et al., Virology, 307:213–227, 2004) suggest that the chimera CP7_E2alf is a potential CSF marker vaccine candidate.
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References
Ahrens U, Kaden V, Drexler Ch, Visser N (2000) Efficacy of the classical swine fever (CSF) marker vaccine Porcilis® Pesti in pregnant sows. Vet Microbiol 77:83–97
Anonymous (2002) A diagnostic manual establishing diagnostic procedures, sampling methods and criteria for evaluation of laboratory tests for the confirmation of classical swine fever. Commission Decision 2002/106/EC, 1 February 2002. Off J L039:71–88
Brauer A, Lange E, Kaden V (2006) Oral immunisation of wild boar against classical swine fever: uptake studies of new baits and investigations on the stability of lyophilised C-strain vaccine. Eur J Wildl Res 52:271–276
De Smit AJ, Bouma A, van Gennip EP, de Kluijver EP, Moormann RJM (2001) Chimeric (marker) C-strain virus induce clinical protection against virulent classical swine fever virus (CSFV) and reduce transmission of CSFV between vaccinated pigs. Vaccine 19:1467–1476
Dong X-N, Wei K, Liu ZQ, Chen YH (2002) Candidate peptide vaccine induced protein against classical swine fever virus. Vaccine 13:167–173
Dong X-N, Qi Y, Ying J, Chen X, Chen Y-H (2006) Candidate peptide-vaccine induced potent protection against CSFV and identified a principal sequential neutralising determinant on E2. Vaccine 24:426–434
Faust A, Lange E, Kaden V (2007) Efficacy of lyophilised C-strain vaccine after oral vaccination of domestic pigs and wild boar: first results. Dtsch Tierärztl Wschr (in press)
Hahn J, Park SH, Song JY, An SH, An BY (2001) Construction of recombinant swinepox viruses and expression of the classical swine fever virus E2 protein. J Virol Methods 93:49–56
Haegeman A, Dewulf J, Vrancken R, Tignon M, Ribbens S, Koenen F (2006) Characterisation of the discrepancy between PCR and virus isolation in relation to classical swine fever virus detection. J Virol Methods 136:44–50
Hammond JM, Jansen ES, Morrissy CJ, Williamson MM, Hodgson ALM, Johnson MA (2001) Oral and sub-cutaneous vaccination of commercial pigs with a recombinant porcine adenovirus expressing the classical swine fever virus gp55 gene. Arch Virol 146:1787–1793
Hammond JM, Jansen ES, Morrissy CJ, Hodgson ALM, Johnson MA (2003) Protection of pigs against ‘in contact’ challenge with classical swine fever following oral or subcutaneous vaccination with a recombinant porcine adenovirus. Virus Res 97:151–157
Hoffmann B, Beer M, Schelp C, Schirrmeier H, Depner K (2005) Validation of a real-time RT-PCR assay for sensitive and specific detection of classical swine fever. J Virol Methods 130:36–44
Kaden V, Lange B (2001) Oral immunisation against classical swine fever: onset and duration of immunity. Vet Microbiol 82:301–310
Kaden V, Steyer H, Schurig U, Strebelow G, Lange E, Hübert P, Steinhagen P (1999) Detection of low-virulent classical swine fever virus in blood of experimentally infected animals: comparison of different methods. Acta Virol 43:373–380
Kaden V, Schurig U, Steyer H (2001) Oral immunization of pigs against classical swine fever. Course of the disease and virus transmission after simultaneous vaccination and infection. Acta Virol 45:23–29
Kaden V, Heyne H, Kiupel H, Letz W, Kern B, Gossger K, Rothe A, Böhme H, Tyrpe P (2002) Oral immunisation of wild boar against classical swine fever: concluding analysis of the recent field trials in Germany. Berl Münch Tierärztl Wschr 115:179–185
Kaden V, Irsch B, Schwickert S, Blicke J, Straubinger B (2003a) State of the art of oral immunisation of wild boar against classical swine fever in Rhineland—Palatinate. Report on Annual Meeting of National Classical Swine Fever Laboratories, Brussels, Belgium, 12–13 May, European Commission, Doc. SANCO/10457/2003, pp 32–39
Kaden V, Renner Ch, Rothe A, Lange E, Hänel A, Gossger K (2003b) Evaluation of the oral immunisation of wild boar against classical swine fever in Baden—Württemberg. Berl Münch Tierärztl Wschr 116:362–367
Kaden V, Lange E, Riebe R, Lange B (2004) Classical swine fever virus strain “C”. How long is it detectable after oral vaccination. J Vet Med B 51:260–262
Kaden V, Lange E, Müller T, Teuffert J, Teifke JP, Riebe R (2006) Protection of gruntlings against classical swine fever virus-infection after oral vaccination of sows with C-strain vaccine. J Vet Med B 53:455–460
Maurer R, Stettler P, Ruggli N, Hofmann MA, Tratschin J-D (2005) Oronasal vaccination with classical swine fever virus (CSFV) replicon particles with either partial or complete deletion of the E2 gene induces partial protection against lethal challenge with highly virulent CSFV. Vaccine 23:3318–3328
Meyers G, Tautz N, Becher P, Thiel HJ, Kümmerer BM (1996) Recovery of cytopathogenic and noncytopathogenic bovine viral diarrhea viruses from cDNA constructs. J Virol 70:8606–8613
Reimann I, Meyers G, Beer M (2003) Trans-complementation of autonomously replicating bovine viral diarrhea virus replicons with deletions in the E2 coding region. Virology 307:213–227
Reimann I, Depner K, Trapp S, Beer M (2004) An avirulent chimeric Pestivirus with altered cell tropism protects pigs against a lethal infection with classical swine fever virus. Virology 322:143–157
Ruggli N, Tratschin J-D, Mittelholzer C, Hofmann MA (1996) Nucleotide sequence of classical swine fever virus strain Alfort/187 and transcription of infectious RNA from stably cloned full-length cDNA. J Virol 70:3478–3487
Rümenapf T, Stark R, Meyers G, Thiel H-J (1991) Structural proteins of hog cholera virus expressed by vaccinia virus: further characterization and introduction of protective immunity. J Virol 65:589–597
Rümenapf T, Unger G, Strauss JH, Thiel HJ (1993) Processing of the envelope glycoproteins of Pestiviruses. J Virol 67:3288–3294
Van Gennip HG, van Rijn PA, Widjojoatmodjo MN, de Smit AJ, Moormann RJ (2000) Chimeric classical swine fever viruses containing envelope protein E(RNS) or E2 of bovine viral diarrhoea virus protect pigs against challenge with CSFV and induce a distinguishable antibody response. Vaccine 19:447–459
Van Oirschot JT (1999) Diva vaccines that reduce virus transmission. J Biotech 73:195–205
Yu X, Tu C, Li H, Hu R, Chen C, Li Z, Zang M, Yin Z (2001) DNA-mediated protection against classical swine fever virus. Vaccine 19:1520–1525
Acknowledgments
The authors thank Anette Beidler for critical review of the manuscript. Furthermore, we acknowledge J.P. Teifke for postmortem investigations as well as G. Strebelow for sequencing of the viral RNA. This study was supported by the EC project “CSF Vaccine & Wild Boar” (no. SSPE-CT-2003-501599). The experiments comply with the current laws and are approved by the ethics commission for animal experiments of the Federal State Mecklenburg—Western Pomerania.
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Communicated by F.-J. Kaup
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Kaden, V., Lange, E. & Faust, A. Oral vaccination against classical swine fever with a chimeric Pestivirus: comparative investigations of liquid and lyophilized virus. Eur J Wildl Res 54, 237–244 (2008). https://doi.org/10.1007/s10344-007-0136-9
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DOI: https://doi.org/10.1007/s10344-007-0136-9