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The Responses of ‘Viking’ Aronia Variety to Salinity Stress under In Vitro Conditions

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Abstract

In this study, the effects of different NaCl levels (0, 50, 100, 150 mM) on ‘Viking’ aronia variety plants in the in vitro culture medium were investigated. In the study, aronia plantlets were cultured in the MS and the ½MS rooting media with 1 mg l−1 IBA supplemented with different concentrations of NaCl for 35 days. At the end of salinity stress treatments, plant height, leaf area, plant fresh and dry weight, rooting rate, root number, root length as well as root fresh and dry weight were measured. In all measurements recorded for salinity stress, there was a decrease in all growth parameters as the NaCl level increased in both media. The ½MS medium compared to the full MS medium performed better at almost all growth parameters at every NaCl concentration. The average highest plant height was recorded in the control treatment (4.77 cm) of the ½MS medium; the lowest plant height was recorded in 150 mM NaCl treatment (1.67 cm) of the MS medium. The lowest leaf area was determined at 150 mM NaCl concentration, and it reduced 68.8% in the MS medium and 73% in the ½MS medium. Rooting was highest in the control groups (100%) in both media; a significant gradual decrease occurred in 50 and 100 mM NaCl concentration, and rooting did not occur in 150 mM NaCl treatment. In addition, with increasing salt concentration in the culture medium, both root length and number decreased. Consequently, it has been determined that the ‘Viking’ aronia variety is sensitive to high salinity levels.

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References

  • Abdel-Hussein M (2005) Evaluation of MM106 and Omara apple rootstocks for salt tolerance in vitro. J Kerbala Univ 1(1):178–185

    Google Scholar 

  • Al-Karaki GN (2006) Nursery inoculation of tomato with arbuscular mycorrhizal fungi and subsequent performance under irrigation with saline water. Sci Hortic 109(1):1–7

    Article  Google Scholar 

  • Anjum MA (2011) Effect of exogenously applied spermidine on growth and physiology of citrus rootstock Troyer citrange under saline conditions. Turk J Agric For 35(1):43–53

    Google Scholar 

  • Bahmani R, Gholami M, Mozafari A‑A, Alivaisi R (2012) Effects of salinity on in vitro shoot proliferation and rooting of apple rootstock MM. 106. World Appl Sci J 17(3):292–295

    CAS  Google Scholar 

  • Brzóska MM, Rogalska J, Roszczenko A, Galazyn-Sidorczuk M, Tomczyk M (2016) The mechanism of the osteoprotective action of a polyphenol-rich Aronia melanocarpa extract during chronic exposure to cadmium is mediated by the oxidative defense system. Planta Med 82(07):621–631

    Article  PubMed  Google Scholar 

  • Chartzoulakis K, Klapaki G (2000) Response of two greenhouse pepper hybrids to NaCl salinity during different growth stages. Sci Hortic 86(3):247–260

    Article  CAS  Google Scholar 

  • D’Agata C, Diolaiuti G, Maragno D, Smiraglia C, Pelfini M (2020) Climate change effects on landscape and environment in glacierized Alpine areas: retreating glaciers and enlarging forelands in the Bernina group (Italy) in the period 1954–2007. Geol Ecol Landscapes 4(1):71–86

    Article  Google Scholar 

  • Ding Y, Ma Y (2020) Hyper spectral image characteristics of aronia melanocarpa leaves under saline alkali stress. Glob Nest J 22(4):603–612

    CAS  Google Scholar 

  • Ecehagh M, Samaneh F, Hadi S, Golale A, Fagheh S, Aliraza S, Mohammad SHG (2012) Effects of salinity stress on growth and yield of Aloe vera L. J Med Plants Res 6(16):3272–3277

    Google Scholar 

  • Engin SP (2020) ‘Nero’ve ‘Viking’aronya (Aronia Melanocarpa (Michx) Elliot) Çeşitlerinin Agromorfolojik Özellikleri Ve Farklı Olgunluk Seviyelerindeki Meyve Kalite Parametrelerinin Belirlenmesi. Bursa Uludag University

    Google Scholar 

  • Epstein E, Rains D (1987) Advances in salt tolerance. In: Genetic aspects of plant mineral nutrition. Springer, Berlin Heidelberg, pp 113–125

    Chapter  Google Scholar 

  • Eraslan F, Arici ŞE, Erdal İ, Küçükyumuk Z (2016) Kiraz Anaçlarının in vitro koşullarda tuz stresine tolerans mekanizmalarının fizyolojik parametreler ve antioksidan enzim izoformları ile belirlenmesi. J Agric Sci 22(1):117–128

    Google Scholar 

  • Ghaleb WS, Sawwan JS, Akash MW, Al-Abdallat AM (2010) In vitro response of two Citrus rootstocks to salt stress. Int J Fruit Sci 10(1):40–53

    Article  Google Scholar 

  • Giri B, Kapoor R, Mukerji K (2003) Influence of arbuscular mycorrhizal fungi and salinity on growth, biomass, and mineral nutrition of Acacia auriculiformis. Biol Fertil Soils 38(3):170–175

    Article  Google Scholar 

  • Haque SA (2006) Salinity problems and crop production in coastal regions of Bangladesh. PAK J BOT 38(5):1359–1365

    Google Scholar 

  • Howat D (2000) Acceptable salinity, sodicity and pH values for boreal forest reclamation. Environmental Sciences Division Edmonton, Edmonton

    Google Scholar 

  • İpek M, Arikan Ş, Pirlak L, Eşitken A (2016) The response of Myrobolan 29c plum rootstock to salinity. In: Vitro culture condition, Proceedings book

    Google Scholar 

  • Litwińczuk W (2012) Micropropagation of chokeberry by in vitro axillary shoot proliferation. In: Protocols for Micropropagation of selected economically-important horticultural plants. Springer, Berlin Heidelberg, pp 179–186

    Chapter  Google Scholar 

  • Lobell DB, Ortiz-Monasterio JI, Gurrola FC, Valenzuela L (2007) Identification of saline soils with multiyear remote sensing of crop yields. Soil Sci Soc Am J 71(3):777–783

    Article  CAS  Google Scholar 

  • Mahajan S, Tuteja N (2005) Cold, salinity and drought stresses: an overview. Arch Biochem Biophys 444(2):139–158

    Article  CAS  PubMed  Google Scholar 

  • Mahmood MS, Pırlak L (2023) Aronya (Aronia melanocarpa) Fidanlarının. In: Vitro ve In Vivo Şartlarda Tuz Stresine Toleranslarının Belirlenmesi International Conference on Scientific and Innovative Studies, pp 86–91

    Google Scholar 

  • Marschner C (1996) Mineral nutrition of higher plants. Academic Press, London, p 889

    Google Scholar 

  • Mobayen R, Milthorpe F (1980) Response of seedlings of three citrus-rootstock cultivars to salinity. Aust J Agric Res 31(1):117–124

    Article  CAS  Google Scholar 

  • Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651

    Article  CAS  PubMed  Google Scholar 

  • Nandal M, Hooda R (2013) Salt tolerance and physiological response of plants to salinity: a review. Int J Sci Eng Res 4(10):44–67

    Google Scholar 

  • Nas Z (2021) Anaçlık Potansiyel Gösteren 42-01 Zerdali Genotipinin. In: Vitro Mikroçoğaltım Özelliklerinin Araştırılması, Yüksek Lisans Tezi. Selçuk Üniversitesi, Konya, p 51

    Google Scholar 

  • Patil V (1978) Growth behaviour of certain citrus rootstocks as influenced by different levels of salinity in soil. Haryana J Hortic Sci 7:105–111

    Google Scholar 

  • Pırlak L, Almokar H (2018) Propagation of Aronia (Aronia melanocarpa) with tissue culture. Selcuk J Agric Food Sci 32(3):549–558

    Google Scholar 

  • Rusea I, Popescu A, Isac V, Șutan A, Hoza D (2019) High efficiency shoot multiplication from in vitro cultured meristems of Aronia Melanocarpa Cv. nero, scientific papers. Series B. Horticulture 63(1):65–74

    Google Scholar 

  • Sharma L, Kaushal M, Bali SK, Choudhary O (2013) Evaluation of rough lemon (Citrus jambhiri Lush.) as rootstock for salinity tolerance at seedling stage under in vitro conditions. Afr J Biotechnol 12(44):6267–6275

    Article  Google Scholar 

  • Šiler B, Mišić D, Filipović B, Popović Z, Cvetić T, Mijović A (2007) Effects of salinity on in vitro growth and photosynthesis of common centaury (Centaurium erythraea Rafn.). Arch Biol Sci 59(2):129–134

    Article  Google Scholar 

  • Tester M, Davenport R (2003) Na+ tolerance and Na+ transport in higher plants. Ann Bot 91(5):503–527

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Trejgell A, Dąbrowska G, Tretyn A (2009) In vitro regeneration of Carlina acaulis subsp. simplex from seedling explants. Acta Physiol Plantarum 31(3):445–453

    Article  Google Scholar 

  • Yurtkulu V (2021) Aronya Fizibilite Raporu ve Yatırımcı Rehberi. Tarım ve Orman Bakanlığı, Bitkisel Üretim Genel Müdürlüğü, Ankara

    Google Scholar 

  • Zidan I, Azaizeh H, Neumann PM (1990) Does salinity reduce growth in maize root epidermal cells by inhibiting their capacity for cell wall acidification? Plant Physiol 93(1):7–11

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Further reading

  • Almokar HMM, Pırlak L (2018) Propagation of Aronia (Aronia melanocarpa) with tissue culture. Selcuk J Agric Sci. https://doi.org/10.15316/SJAFS.2018.136

    Article  Google Scholar 

  • Binh DQ, Heszky LE, Gyulai G, Csillag A (1992) Plant regeneration of NaCl-pretreated cells from long-term suspension culture of rice (Oryza sativa L.) in high saline conditions. Plant Cell Tiss Organ Cult 29(2):75–82

    Article  Google Scholar 

  • Hartmann HT, Kester DE, Davies FT, Geneve RL (1997) Plant propagation: principles and practices, 6th edn. Prentice-Hall, Hoboken

    Google Scholar 

  • Khan MI, Khan MA, Khizar T (1976) Plant growth regulators from species differing in salt tolerance as affected by soil salinity. Plant Soil 45(1):267–271

    Article  CAS  Google Scholar 

  • Varshney RK, Bansal KC, Aggarwal PK, Datta SK, Craufurd PQ (2011) Agricultural biotechnology for crop improvement in a variable climate: hope or hype? Trends Plant Sci 16(7):363–371

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Horticulture, Selcuk University, Konya, Turkey

    Zeynep Nas, Ahmet Eşitken & Lütfi Pirlak

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  1. Zeynep Nas
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  2. Ahmet Eşitken
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  3. Lütfi Pirlak
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Correspondence to Zeynep Nas.

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Z. Nas, A. Eşitken, and L. Pirlak declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Nas, Z., Eşitken, A. & Pirlak, L. The Responses of ‘Viking’ Aronia Variety to Salinity Stress under In Vitro Conditions. Erwerbs-Obstbau 65, 2547–2552 (2023). https://doi.org/10.1007/s10341-023-00954-0

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