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Layer-Induced Sensitivity Enhancement in Planar Chromatography–Bioluminescence–Mass Spectrometry: Application to Alkaloids

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Abstract

The use of amino phases induced a significant increase of the bioluminescence for the high-performance thin-layer chromatography (HPTLC)-Aliivibrio fischeri bioassay. By this, the detectability of alkaloids was improved. Detection limits at the low ng per zone level were obtained for the five alkaloids berberine, palmatine, ephedrine, norephedrine, and methylephedrine. The influence of other parameters on this effect, such as fluorescence indicators and pH milieu, was proven to be insignificant. This implied that propyl amino groups linked to the silica gel layer might play a pronounced role in the improved visualization mechanism. The optimized bioassay was applied for profiling of alkaloid-rich herbal drugs such as Philodendron, Coptis, Tinospora, and Ephedra, leading to information-rich biofingerprints. The direct coupling of HPTLC to electrospray ionization mass spectrometry enabled unambiguous and straightforward confirmation of the bioactive ingredients.

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Acknowledgments

Chen Yisheng was financially supported by the China Scholarship Council and Jiangnan University, Wuxi, China (research grant numbers: 2012BAD37B06, 2012BAD37B07, and JUDCF10049), for which the support by Prof. Dr. Xu Xueming and ** Zhengyu is acknowledged. Thank is also owed to Tim Häbe, Justus Liebig University Giessen, Germany, for instrumental support.

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Authors and Affiliations

  1. Chair of Food Science, Interdisciplinary Research Center (IFZ), Institute of Nutritional Science, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany

    Yisheng Chen & Gertrud E. Morlock

  2. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Yisheng Chen

Authors
  1. Yisheng Chen
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  2. Gertrud E. Morlock
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Correspondence to Gertrud E. Morlock.

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Y. Chen: on leave from School of Food Science and Technology, Jiangnan University, 214022 Wuxi, China.

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Chen, Y., Morlock, G.E. Layer-Induced Sensitivity Enhancement in Planar Chromatography–Bioluminescence–Mass Spectrometry: Application to Alkaloids. Chromatographia 79, 89–96 (2016). https://doi.org/10.1007/s10337-015-2994-8

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