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Bioanalysis of Cepharanthine by LC–ESI–MS–MS and Its Application to Pharmacokinetic Studies

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Abstract

A sensitive liquid chromatography–electrospray ionization–tandem mass spectrometry (LC–ESI–MS–MS) method was developed and validated for the quantification of cepharanthine (CEP) in beagle dog plasma. The chromatographic separation was performed on an Agilent-C18 column and the mobile phase was composed of methanol:water with 10 mM ammonium acetate (20:80, v/v). Detection was operated in the positive ion mode and the tandem mass spectrometer was tuned in the multiple reactions monitoring mode (MRM) to monitor m/z transitions 607 → 365 for CEP and 285 → 193 for the internal standard (IS) diazepam. This method exhibited a linear range of 5–2,500 ng mL−1. The precision (RSD%) and accuracy (RME%) of the assay were <8.7 and 2.4%, respectively. The limit of quantification was 5 ng mL−1 and no significant matrix effect was observed. The validated method has been successfully applied to pharmacokinetic study of CEP in beagle dog.

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Acknowledgments

This work was supported by Professor Qing-Duan Wang and Department of Pharmacology, Henan Institute of Medical Sciences.

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Authors and Affiliations

  1. Department of Clinical Pharmacology, Affiliated Hospital, Academy of Military Medical Sciences, Bei**g, 100071, China

    Rui-Hua Dong, Hong-Zhi Gao, Guang-Tao Hao, Guang Liu, Ting-Ting Shan & Ze-Yuan Liu

  2. Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China

    Zhong-Ze Fang

  3. Graduate School of Chinese Academy of Sciences, Bei**g, 100049, China

    Zhong-Ze Fang

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  1. Rui-Hua Dong
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  2. Zhong-Ze Fang
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  3. Hong-Zhi Gao
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  4. Guang-Tao Hao
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  5. Guang Liu
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  7. Ze-Yuan Liu
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Correspondence to Ze-Yuan Liu.

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Dong, RH., Fang, ZZ., Gao, HZ. et al. Bioanalysis of Cepharanthine by LC–ESI–MS–MS and Its Application to Pharmacokinetic Studies. Chromatographia 73, 75–81 (2011). https://doi.org/10.1007/s10337-010-1823-3

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  • DOI: https://doi.org/10.1007/s10337-010-1823-3

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