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Complementary DNA Cloning and Molecular Evolution of Opine Dehydrogenases in Some Marine Invertebrates

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Abstract

The complete complementary DNA sequences of genes presumably coding for opine dehydrogenases from Arabella iricolor (sandworm), Haliotis discus hannai (abalone), and Patinopecten yessoensis (scallop) were determined, and partial cDNA sequences were derived for Meretrix lusoria (Japanese hard clam) and Spisula sachalinensis (Sakhalin surf clam). The primers ODH-9F and ODH-11R proved useful for amplifying the sequences for opine dehydrogenases from the 4 mollusk species investigated in this study. The sequence of the sandworm was obtained using primers constructed from the amino acid sequence of tauropine dehydrogenase, the main opine dehydrogenase in A. iricolor. The complete cDNA sequence of A. iricolor, H. discus hannai, and P. yessoensis encode 397, 400, and 405 amino acids, respectively. All sequences were aligned and compared with published databank sequences of Loligo opalescens, Loligo vulgaris (squid), Sepia officinalis (cuttlefish), and Pecten maximus (scallop). As expected, a high level of homology was observed for the cDNA from closely related species, such as for cephalopods or scallops, whereas cDNA from the other species showed lower-level homologies. A similar trend was observed when the deduced amino acid sequences were compared. Furthermore, alignment of these sequences revealed some structural motifs that are possibly related to the binding sites of the substrates. The phylogenetic trees derived from the nucleotide and amino acid sequences were consistent with the classification of species resulting from classical taxonomic analyses.

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Acknowledgments

We thank the Deutsche Forschungsgemeinschaft for providing ongoing and substantial help (M.K.G. and F.J. received a stipend from the Graduiertenkolleg “Molekulare Physiologie: Stoffund Energietransformationen”).

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Authors and Affiliations

  1. Laboratory of Marine Biochemistry, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, 981-8555, Japan

    Tomohiro Kimura, Toshiki Nakano, Toshiyasu Yamaguchi & Minoru Sato

  2. Graduate School of Life Sciences, Tohoku University, Aoba-ku, Sendai, 981-8555, Japan

    Tomohisa Ogawa & Koji Muramoto

  3. School of Fisheries Science, Kitasato University, Oofunato, Iwate, 022-0101, Japan

    Takehiko Yokoyama, Nobuhiro Kan-no & Eizou Nagahisa

  4. Institut für Zoophysiologie, Heinrich-Heine-Universität, Universitätsstrasse 1, Düsseldorf, 40225, Germany

    Frank Janssen & Manfred K. Grieshaber

Authors
  1. Tomohiro Kimura
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  2. Toshiki Nakano
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  3. Toshiyasu Yamaguchi
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  4. Minoru Sato
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  5. Tomohisa Ogawa
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  6. Koji Muramoto
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  7. Takehiko Yokoyama
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  8. Nobuhiro Kan-no
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  9. Eizou Nagahisa
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  10. Frank Janssen
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  11. Manfred K. Grieshaber
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Corresponding author

Correspondence to Minoru Sato.

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Kimura, T., Nakano, T., Yamaguchi, T. et al. Complementary DNA Cloning and Molecular Evolution of Opine Dehydrogenases in Some Marine Invertebrates. Mar Biotechnol 6, 493–502 (2004). https://doi.org/10.1007/s10126-004-2700-6

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  • DOI: https://doi.org/10.1007/s10126-004-2700-6

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