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Detection of human JCPyV and BKPyV in diffuse large B-cell lymphoma of the GI tract

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Abstract

Previous studies have demonstrated that infection with human polyomavirus, such as JCPyV and BKPyV, might be associated with various human tumors. However, an association between human JCPyV and BKPyV infection and diffuse large B-cell lymphoma (DLBCL) has not been reported. The purpose of this study was to examine DLBCLs of the gastrointestinal tract for evidence of human polyomavirus infection. Nested PCR and DNA sequencing were employed for viral DNA detection and viral genotype identification. In addition, two viral proteins, the large tumor antigen (LT) and the major structural protein (VP1), were detected by immunohistochemistry (IHC). Human JCPyV and BKPyV DNA was detected in 14 out of 16 tissue samples (87.5 %), whereby nine cases were infected with JCPyV and five cases were infected with BKPyV. Both archetypal and rearranged genotypes of JCPyV and BKPyV were detected in the tissues. LT was detected in 11 tissue samples (68.75 %). However, VP1 was not detected in any of the tissue samples. The presence of human JCPyV and BKPyV DNA and protein in DLBCL tissues of gastrointestinal tract were first reported in this study. The current results provide evidence of a possible association between human JCPyV and BKPyV infection and DLBCL.

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Acknowledgments

This work was supported by research grants from the National Science Council, NSC 101-2320-B-194-002-MY3, Dalin Tzu Chi General Hospital at Chia-Yi, DTCRD96(2)-04 and Chung Shan Medical University, CSMU-INT-101-13, Taiwan, ROC. The upright fluorescence microscope was performed in the Instrument Center of Chung Shan Medical University, which was supported by the National Science Council, Ministry of Education and Chung Shan Medical University.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Anatomic Pathology, Buddhist Dalin Tzu Chi General Hospital, Chiayi, Taiwan

    C. E. Tseng

  2. School of Medicine, Tzu Chi University, Hualien, Taiwan

    C. E. Tseng

  3. Institute of Molecular Biology, National Chung Cheng University, Chiayi, Taiwan

    C. M. Yeh, J. Shay, M. C. Lin & D. Chang

  4. Department of Medical Research, Chiayi Christian Hospital, Chiayi, Taiwan

    C. Y. Fang

  5. Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung, Taiwan

    P. L. Chen

  6. Department of Urology, Chiayi Christian Hospital, Chiayi, Taiwan

    M. C. Lin

  7. Department of Microbiology and Immunology, Clinical Research Center, Chung Shan Medical University Hospital, Chung Shan Medical University, Taichung, Taiwan

    M. Wang

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Correspondence to M. Wang.

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Tseng, C.E., Yeh, C.M., Fang, C.Y. et al. Detection of human JCPyV and BKPyV in diffuse large B-cell lymphoma of the GI tract. Eur J Clin Microbiol Infect Dis 33, 665–672 (2014). https://doi.org/10.1007/s10096-013-2010-x

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