Abstract
Buckwheat germinated on days of 3, 5, and 7 was digested in vitro, and the antioxidant and anti-obesity effects of the digesta were evaluated. In vitro digesta of 5 days germinated buckwheat (GBD5) showed significantly higher antioxidant activity in DPPH, ABTS, total phenolic content, total flavonoid content, and ferric reducing antioxidant power by 5.3, 1.3, 2.0, 3.2, and 2.8-fold, respectively than in vitro digesta of non-germinated buckwheat. GBD5 exerted inhibitory effect on total lipid accumulation in 3T3-L1 adipocyte in a dose-dependent manner, with over 25% reduction at 400 µg/mL. Additionally, GBD5 significantly downregulated genes related to adipocyte differentiation and fat accumulation. GBD5 possessed different metabolite profiles compared to others such as higher content of γ-aminobutyric acid and succinic acid. Therefore, GBD5 has potent antioxidant effects and suppresses fat accumulation-related genes and proteins expression, which could act as a new functional substance.
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This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2018R1D1A3B07045467).
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Bae, HG., Kim, MJ. Antioxidant and anti-obesity effects of in vitro digesta of germinated buckwheat. Food Sci Biotechnol 31, 879–892 (2022). https://doi.org/10.1007/s10068-022-01086-z
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DOI: https://doi.org/10.1007/s10068-022-01086-z