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Adjuvant effect of Korean mistletoe lectin on mucosal immunity induction following intranasal immunization with hemagglutinin antigen

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Abstract

To determine Korean mistletoe lectin (KML-C) functions as a mucosal adjuvant, we monitored its ability to modulate the immune response after intranasal immunization with hemagglutinin (HA) as an antigen. Mucosal and systemic immune responses with KML-C were compared to responses with cholera toxin B subunit (CTB) as an adjuvant. Intranasal co-administration of HA and KML-C in mice resulted in increased anti-HA antibody titers in both vaginal wash and serum. Isoty** analysis revealed that KML-C augmented HA-specific antibody titers of IgG1, IgG2a, and IgG2b isotypes. More than 5-fold of interleukin 2 (IL-2), 3-fold of interferon-γ (IFN-γ), and 4-fold of granulocyte macrophage colony-stimulating factor (GM-CSF) mRNA were detected using real time PCR in splenocytes of mice immunized with HA plus KML-C. Fluorescence activated cell sorting (FACS) analysis indicated that co-administration of KML-C increased the subpopulation of CD3/CD8 double positive T cells (16.4%) than control (10%). Taken together, these data demonstrate that KML-C has the ability to serve as a mucosal adjuvant.

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Authors and Affiliations

  1. School of Life Sciences, Handong Global University, Pohang, Gyeongbuk, 791-708, Korea

    ** Hyuk Jung, Young Hoon Kim, Tae Jun Song, Hyosun An, Kyu Dae Kim, In Bo Kim & Jong Bae Kim

  2. Department of Food and Nutrition, Yuhan University, Bucheon, Gyeonggi, 422-749, Korea

    Taek Joon Yoon

  3. Functional Genoproteome Research Centre, Konkuk University, Seoul, 143-701, Korea

    ** Hyuk Jung

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  1. ** Hyuk Jung
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Correspondence to Jong Bae Kim.

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Jung, J.H., Kim, Y.H., Song, T.J. et al. Adjuvant effect of Korean mistletoe lectin on mucosal immunity induction following intranasal immunization with hemagglutinin antigen. Food Sci Biotechnol 20, 629–634 (2011). https://doi.org/10.1007/s10068-011-0089-3

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  • DOI: https://doi.org/10.1007/s10068-011-0089-3

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