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Radiation-induced gliomas: a report of four cases and analysis of molecular biomarkers

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Abstract

Radiation-induced glioma (RIG) is a rare secondary glioma. The tumors morphologically resemble their sporadically arising counterparts. Recently, the WHO classification of tumors of the central nervous system was revised to incorporate molecular biomarkers together with classic histological features. The status of molecular biomarkers in RIG, however, remains unclear. The objective of this study was to investigate if commonly accepted glioma-specific biomarkers are relevant in RIGs. Among 269 gliomas diagnosed as WHO grade 2, 3 and 4 in our institution, four were diagnosed as RIGs. Immunohistochemical (IHC) staining for isocitrate dehydrogenase 1 (IDH1), p53, alpha thalassemia/mental retardation syndrome X-linked (ATRX), and H3K27M, and direct DNA sequencing of IDH1/2, telomerase reverse transcriptase (TERT) promoter, Histone H3.3 (H3F3A) and B-Raf (BRAF) genes was performed. All tumor specimens were IDH1-, p53- and H3K27M-negative. The nuclei of tumor cells in all cases exhibited positive staining for ATRX. In direct DNA sequencing analysis, no IDH1, IDH2, TERT promoter, H3F3A or BRAF mutations were found in any of the cases. Our findings suggest that these characteristic glioma-associated molecular mutations may be rare events in RIGs. More RIGs need to be tested for analysis of molecular biomarkers to clarify the clinical and histopathological spectra of this tumor.

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Acknowledgements

The authors wish to thank Ms. Erika Komura for her invaluable assistance with the pathological study.

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Authors and Affiliations

  1. Division of Neuroscience, Department of Neurosurgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa, 920-8641, Japan

    Tsunehito Nakao, Yasuo Sasagawa, Hemragul Sabit, Masashi Kinoshita, Katsuyoshi Miyashita, Yasuhiko Hayashi & Mitsutoshi Nakada

  2. Department of Human Pathology, Gunma University Graduate School of Medicine, Gunma, Japan

    Sumihito Nobusawa & Hideaki Yokoo

  3. Department of Neurosurgery, Fukui-ken Saiseikai Hospital, Fukui, Japan

    Yasushi Takabatake

Authors
  1. Tsunehito Nakao
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  2. Yasuo Sasagawa
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  3. Sumihito Nobusawa
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  4. Yasushi Takabatake
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  10. Mitsutoshi Nakada
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Correspondence to Yasuo Sasagawa.

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Nakao, T., Sasagawa, Y., Nobusawa, S. et al. Radiation-induced gliomas: a report of four cases and analysis of molecular biomarkers. Brain Tumor Pathol 34, 149–154 (2017). https://doi.org/10.1007/s10014-017-0292-x

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  • DOI: https://doi.org/10.1007/s10014-017-0292-x

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