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Electrochemical detection of creatinine using Au–Ag bimetallic nanoparticles

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Abstract

The adsorption of creatinine has been examined at Au–Ag bimetallic nanoparticles. In phosphate buffer without a supporting electrolyte, both metals were oxidised within a single voltammetric peak. The oxidation peak height was inversely proportional to the creatinine concentration, indicating adsorption of the species onto the particles. The adsorption followed a Langmuir isotherm with a free energy of adsorption estimated at − 14.4 kJ mol−1. The adsorption was found to be selective in the presence of interferents such as glucose, glycine, and urea. Using the decrease in peak height creatinine could be calibrated with an LOD of 0.8 mM (defined as 3.3σ/S where σ is the standard deviation of the blank and S is the calibration gradient) and a sensitivity of 137.0 µA mM−1 (r2 = 0.998). Recoveries of creatinine in artificial urine were in the range 92.8 to 104.5%.

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Data Availability

Data recorded in this study are available from the corresponding author on reasonable request.

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Acknowledgements

A. N. is grateful for a post-doctoral fellowship from King Mongkut’s University of Technology Thonburi.

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Authors and Affiliations

  1. Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkhuntien-Chaitalay Road, Thakam, Bangkok, 10150, Thailand

    A**kya Nene & Werasak Surareungchai

  2. Materials and Interfaces Center, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China

    A**kya Nene

  3. School of Bioresources and Technology, Nanoscience & Nanotechnology Graduate Programme, and, Faculty of Science , King Mongkut’s University of Technology Thonburi, Bangkhuntien-Chaitalay Road, Thakam, Bangkok, 10150, Thailand

    Werasak Surareungchai

  4. Analytical Sciences and National Do** Test Institute, Mahidol University, Bangkok, 10400, Thailand

    Werasak Surareungchai

  5. Biosciences and System Biology Team Biochemical Engineering and System Biology Research Group National Center for Genetic Engineering and Biotechnology National Science and Technology Development Agency at KMUTT (Bangkhuntien Campus, Bangkok, 10150, Thailand

    Chatuporn Phanthong & Mithran Somasundrum

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  1. A**kya Nene
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Nene, A., Phanthong, C., Surareungchai, W. et al. Electrochemical detection of creatinine using Au–Ag bimetallic nanoparticles. J Solid State Electrochem 27, 2869–2875 (2023). https://doi.org/10.1007/s10008-023-05571-1

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  • DOI: https://doi.org/10.1007/s10008-023-05571-1

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