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Evansella halocellulosilytica sp. nov., an alkali-halotolerant and cellulose-dissolving bacterium isolated from bauxite residue

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Abstract

An alkali and salt-tolerating strain FJAT-44876T was isolated from the bauxite residue sample. The 16S rRNA gene sequence and phylogenetic analysis suggest that strain FJAT-44876T was a member of the genus Evansella. It grew at 15–45 ℃ (optimum 20–25 ℃) and pH 6.5–11.0 (optimum pH 8.0–9.0) with 0–20% (w/v) NaCl (optimum 6–8%). The major fatty acids were anteiso-C15:0, iso-C15:0, anteiso-C17:0, iso-C17:0, and C16:0. The cell wall peptidoglycan contained meso-diaminopimelic acid and MK-7 as the menaquinone. The major polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, and phosphatidylglycerol. The genomic DNA G+C content was 38.2%. The average nucleotide identity values between strain FJAT-44876T and closely related members were below the cutoff level for species delineation. Thus, based on the above results, strain FJAT-44876T represents a novel species of the genus Evansella, for which the name Evansella halocellulosilytica sp. nov., is proposed. The type strain is FJAT-44876T (=CCTCC AB 2016264T = DSM 104633T).

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Acknowledgements

This is supported by the Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) (Nos 311021004, 311021006), the project of 5511 coordinative innovation (XTCXGC2021019) and Fujian Special Fund for Scientific Research Institutes in the Public Interest (2019R1034-10). The authors are grateful to Prof. Aharon Oren (The Hebrew University of Jerusalem, Israel) for hel** with the etymology.

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  1. Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, Fujian, People’s Republic of China

    Guo-Hong Liu, Qian-Qian Chen, Jian-Mei Che, Huai Shi & Bo Liu

  2. State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Manik Prabhu Narsing Rao & Wen-Jun Li

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Liu, GH., Narsing Rao, M.P., Chen, QQ. et al. Evansella halocellulosilytica sp. nov., an alkali-halotolerant and cellulose-dissolving bacterium isolated from bauxite residue. Extremophiles 26, 19 (2022). https://doi.org/10.1007/s00792-022-01267-y

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