Abstract
Two extremely halophilic archaea, isolates SYSU A00711T and SYSU A00630, were isolated from a sediment soil sample collected from the Aiding lake, China. Cells of these isolates were cocci, non-motile and stained Gram-negative. They grew optimally at 37 °C, with 20–22% NaCl (w/v) and at pH 7.5–8.0. Cells lysed in distilled water. Major polar lipids were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, mannosyl glucosyl diether, sulfated mannosyl glucosyl diether, and two unidentified glycolipids. Pairwise sequence comparison revealed that isolates SYSU A00711T and SYSU A00630 were closely related to Halegenticoccus soli SYSU A9-0T (94.1 and 94.0% 16S rRNA gene sequence similarities; 94.0 and 94.2% rpoB' gene similarities, respectively). The overall genomic relatedness indices values between the two isolates and Halegenticocus soli SYSU A9-0 T were: AAI, both 79.6%; ANI, 84.6 and 84.5%; dDDH, 32.5 and 26.3%, respectively. Phylogenetic trees based on the 16S rRNA gene, rpoB' gene, and genome sequences demonstrated a robust clade of these two isolates with Halegenticoccus soli SYSU A9-0T. The DNA G + C contents of these two isolates are both 64.7% (genome method). Based on the differences in phenotypic, chemotaxonomic, and phylogenetic properties, isolates SYSU A00711T and SYSU A00630 are characterized to represent a novel species in the genus Halegenticoccus, for which the name Halegenticoccus tardaugens sp. nov. is proposed. The type strain of the species Halegenticoccus tardaugens is SYSU A00711T (= KCTC 4245T = CGMCC 1.15768T).
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Notes
The rpoB' gene sequences of isolates SYSU A00711T and SYSU A00630 have been deposited in the GenBank/EMBL/DDBJ with the accession numbers MN592642 and MN592641, respectively. The whole-genome shotgun project data of isolates SYSU A00711T and SYSU A00630 have been deposited at DDBJ/ENA/GenBank under the accessions SDIC00000000 and VCAT00000000, respectively
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Acknowledgements
The authors are grateful to Prof. Yu-Guang Zhou (CGMCC, China) for providing the reference strains. We are also grateful to Mr. Yuan-Guo **e (Sun Yat-sen University, China) for help in generating the phylogenomic tree, and Mr. Zheng-Han Lian and Dr. Jian-Yu Jiao (Sun Yat-sen University, China) for drawing the AAI heatmap.
Funding
This research was supported by the National Natural Science Foundation of China (31800001, 31850410475), Key Scientific Research Project of Colleges and Universities in Henan Province (20A180019), Key Technologies R&D Program of Henan Province (212102310232), Science and Technology Development Special Fund Program of Guangdong Province (2017A030310206) and Key Technologies R&D Program of Nanyang city (KJGG079).
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Liu, BB., Salam, N., Cheng, S. et al. Halegenticoccus tardaugens sp. nov., an extremely halophilic archaeon isolated from a saline soil. Extremophiles 25, 483–492 (2021). https://doi.org/10.1007/s00792-021-01243-y
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DOI: https://doi.org/10.1007/s00792-021-01243-y