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Comparative analysis of trehalose production by Debaryomyces hansenii and Saccharomyces cerevisiae under saline stress

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Abstract

The comparative analysis of growth, intracellular content of Na+ and K+, and the production of trehalose in the halophilic Debaryomyces hansenii and Saccharomyces cerevisiae were determined under saline stress. The yeast species were studied based on their ability to grow in the absence or presence of 0.6 or 1.0 M NaCl and KCl. D. hansenii strains grew better and accumulated more Na+ than S. cerevisiae under saline stress (0.6 and 1.0 M of NaCl), compared to S. cerevisiae strains under similar conditions. By two methods, we found that D. hansenii showed a higher production of trehalose, compared to S. cerevisiae; S. cerevisiae active dry yeast contained more trehalose than a regular commercial strain (S. cerevisiae La Azteca) under all conditions, except when the cells were grown in the presence of 1.0 M NaCl. In our experiments, it was found that D. hansenii accumulates more glycerol than trehalose under saline stress (2.0 and 3.0 M salts). However, under moderate NaCl stress, the cells accumulated more trehalose than glycerol. We suggest that the elevated production of trehalose in D. hansenii plays a role as reserve carbohydrate, as reported for other microorganisms.

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Acknowledgements

This work was partially supported by grant no. 223999 (PAPIIT) from DGAPA, Universidad Nacional Autónoma de México, and no. 36070 N from the Consejo Nacional de Ciencia y Tecnología de México (CONACYT). Juan Carlos González-Hernández was a CONACYT fellow (127608). The authors thank José Sampedro for his comments in this work and the technical assistance of Martha Calahorra and Norma S. Sánchez.

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Authors and Affiliations

  1. Departamento de Genética y Biología Molecular, Instituto de Fisiología celular, Universidad Nacional Autónoma de México, Apartado 70-242, 04510, México D. F., México

    J. C. González-Hernández & A. Peña

  2. Departamento de Productos Naturales, Instituto de Química, Universidad Nacional Autónoma de México, México D. F., México

    M. Jiménez-Estrada

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  1. J. C. González-Hernández
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  2. M. Jiménez-Estrada
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  3. A. Peña
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Correspondence to J. C. González-Hernández.

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Communicated by W.D. Grant

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González-Hernández, J.C., Jiménez-Estrada, M. & Peña, A. Comparative analysis of trehalose production by Debaryomyces hansenii and Saccharomyces cerevisiae under saline stress. Extremophiles 9, 7–16 (2005). https://doi.org/10.1007/s00792-004-0415-2

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