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Trace assay of insulin in a pharmacy drug with a paste electrode

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Abstract

This paper describes the development of a voltammetric assay of insulin using a DNA immobilized onto a carbon nanotube paste electrode (CNPE), the peak potential of which was 0.2 V, vs. Ag/AgCl on the CNPE. The cyclic voltammetry (CV) and square-wave (SW) strip** voltammetry parameters of the optimized conditions were determined. Low analytical working ranges of 10–80 ugL−1 CV and 0.01–0.1 ngL−1 SW were attained. The precision of the insulin concentration of 0.01 ugL−1 was 0.14 (n = 15) RSD using the optimum conditions, in which the detection limit was 0.004 ngL−1 (6.9 × 10–12 M) (S/N = 3) using only an accumulation time of 400 s. The developed method was applied to determine insulin in a pharmacy drug from analytical-grade chemicals (from Aldrich).

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Authors and Affiliations

  1. Department of Anesthesiology and Pain Medicine, Korea University Anam Hospital, 145 Anam ro, Seong buk gu Seoul, Seoul, 02841, South Korea

    In Hea Cho

  2. Sahmyook University, 815 Hwarang ro, Nowon gu, Seoul, 01795, Republic of Korea

    Kwang ** Choi & Jongwan Choi

  3. Biosensor Research Institute, Seoul National University of Science and Technology, 232, no won gu, Seoul, 01811, South Korea

    Kyung Lee & Suw Young Ly

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  1. In Hea Cho
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  2. Kwang ** Choi
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  3. Jongwan Choi
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  4. Kyung Lee
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  5. Suw Young Ly
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Correspondence to Suw Young Ly.

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Cho, I.H., Choi, K.J., Choi, J. et al. Trace assay of insulin in a pharmacy drug with a paste electrode. Amino Acids 55, 1279–1284 (2023). https://doi.org/10.1007/s00726-022-03172-2

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  • DOI: https://doi.org/10.1007/s00726-022-03172-2

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