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Computational image analysis of the baseplate-tail complex of O1 ElTor vibriophage M4

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Abstract

We performed an in-depth computational image analysis of the baseplate-tail complex of the M4 vibriophage and identified seven major densities in its baseplate, which notably share structural similarities with baseplate modules of a number of other bacteriophages belonging to different species. Employing computational analysis, we explained the helical organization of the sheath protein, wrap** the tail tube. Based on the results obtained in this work along with the proteomics information published previously, we are able to decipher the plausible roles assigned to the different components of the M4 baseplate during infection of the host.

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Acknowledgements

The authors declare no conflicts of interest. No animal or human experiments were involved in this work. The authors thank Dr. Moumita Dutta for valuable input. AS and ANG designed the project. AS carried out the computational image analysis. SD carried out proteomics studies. AS and ANG wrote the paper.

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  1. Anindito Sen

    Present address: Microscopy and Imaging Center, Texas A&M University, 301 Old Main Dr., College Station, TX, 77843-2257, USA

Authors and Affiliations

  1. Division of Electron Microscopy, ICMR-National Institute of Cholera and Enteric Diseases, P-33 C.I.T Road, Scheme-XM, Beleghata, Kolkata, 700010, India

    Anindito Sen, Sayani Das & Amar N. Ghosh

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  1. Anindito Sen
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  2. Sayani Das
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  3. Amar N. Ghosh
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Correspondence to Anindito Sen or Amar N. Ghosh.

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Sen, A., Das, S. & Ghosh, A.N. Computational image analysis of the baseplate-tail complex of O1 ElTor vibriophage M4. Arch Virol 165, 2641–2646 (2020). https://doi.org/10.1007/s00705-020-04765-6

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