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Optimized nested PCR enhances biological diagnosis and phylogenetic analysis of human parvovirus B19 infections

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Abstract

Diagnosis and epidemiological analysis of human parvovirus B19 (hB19V) infections are essential for disease management in severely ill patients. This study aimed to evaluate the performance of an optimized NS1-VP1u nested PCR for detection and sequencing of viruses in clinical samples using 224 clinical and five reference samples. PCR sensitivity, specificity, and positive and negative predictive values were perfect (100%). While phylogenetic analysis of a 615 bp-long fragment demonstrated that the viruses in all of the samples belonged to genotype 1, this study confirmed that this optimized PCR could detect all known hB19V with high performance.

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Acknowledgements

The authors want to thank all the technicians of the molecular biology and virology laboratories for their help in these experiments, and Dr. Servant’s laboratory for help in providing the reference samples. The authors want to thank Ms. Viatte, Jeffrey Ashram, and Bruno Simon for valuable advice and correction.

Funding

This research did not receive any specific Grant from funding agencies in the public, commercial or not-for-profit sectors.

Author information

Authors and Affiliations

  1. Hospices Civils de Lyon, Laboratoire de Virologie, Institut des Agents Infectieux, Centre de Biologie et de Pathologie Nord, 103, grande rue de la Croix-Rousse, 69 317, Lyon cedex 04, France

    Maxime Pichon, Clément Labois, Jean-Sébastien Casalegno, Geneviève Billaud, Bruno Lina & Yahia Mekki

  2. Université Lyon 1 Claude Bernard, CIRI, Virpath, Inserm U1111-CNRS UMR 5308, ENS, Lyon, France

    Maxime Pichon, Jean-Sébastien Casalegno & Bruno Lina

  3. Hospices Civils de Lyon, Laboratoire d’Endocrinologie Moléculaire, Centre de Biologie et de Pathologie Est, Bron, France

    Véronique Tardy-Guidollet & Delphine Mallet

  4. Hospices civils de Lyon, Service de Gynécologie-Obstétrique, Hôpital Femme-Mère-Enfant, Bron, France

    Pascal Gaucherand

Authors
  1. Maxime Pichon
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  2. Clément Labois
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  3. Véronique Tardy-Guidollet
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  4. Delphine Mallet
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  5. Jean-Sébastien Casalegno
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  6. Geneviève Billaud
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  7. Bruno Lina
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  8. Pascal Gaucherand
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  9. Yahia Mekki
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Contributions

Conceptualization, MP and CL; data curation, VTG; formal analysis, MP, CL and DM; funding acquisition, BL; investigation, VTG and PG; methodology, MP, CL and JSC; project administration, GB; software, CL; supervision, YM; Writing – original draft, MP; writing – review & editing, MP.

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Correspondence to Maxime Pichon.

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The authors declare that they have no conflict of interest.

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Pichon, M., Labois, C., Tardy-Guidollet, V. et al. Optimized nested PCR enhances biological diagnosis and phylogenetic analysis of human parvovirus B19 infections. Arch Virol 164, 2775–2781 (2019). https://doi.org/10.1007/s00705-019-04368-w

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  • DOI: https://doi.org/10.1007/s00705-019-04368-w

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