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Hybrid nanoflower-based electrochemical lateral flow immunoassay for Escherichia coli O157 detection

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Abstract

An electrochemical biosensor has been developed for detection of Escherichia coli O157 by integrating lateral flow with screen-printed electrodes. The screen-printed electrodes were attached under the lateral flow detection line, and organic–inorganic nanoflowers prepared from E. coli O157-specific antibodies as an organic component were attached to the lateral flow detection line. In the presence of E. coli O157, an organic–inorganic nanoflower-E. coli O157-antimicrobial peptide-labelled ferrocene sandwich structure is formed on the lateral flow detection line. Differential pulse voltammetry is applied using a smartphone-based device to monitor ferrocene on the detection line. The resulting electrochemical biosensor could specifically detect E. coli O157 with a limit of detection of 25 colony-forming units mL−1. Through substitution of antibodies of organic components in organic–inorganic nanoflowers, biosensors have great potential for the detection of other pathogens in biomedical research and clinical diagnosis.

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Funding

This work was financially supported by the Science and Technology Development Plans of Jilin Province (20230203143SF) and the Education Department of Jilin Province (JJKH20210368KJ).

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Authors and Affiliations

  1. College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China

    Jiaqi Wei & Guijuan Qu

  2. Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, 130122, China

    Shengjun Bu, Hongyu Zhou, He Sun, Zhuo Hao & Jiayu Wan

  3. School of Life Science and Technology, Changchun University of Science and Technology, Changchun, 130022, China

    Shengjun Bu

Authors
  1. Jiaqi Wei
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  3. Hongyu Zhou
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  4. He Sun
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  5. Zhuo Hao
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  6. Guijuan Qu
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Contributions

Jiaqi Wei: Experiments, methodology, data curation, writing—original draft. Shengjun Bu: Conceptualization, methodology. Hongyu Zhou: Validation. He Sun: Investigation. Zhuo Hao: Investigation. Guijuan Qu: Conceptualization, methodology, statistical analysis, supervision. Jiayu Wan: Conceptualization; project administration; writing, review; supervision.

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Correspondence to Guijuan Qu or Jiayu Wan.

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Wei, J., Bu, S., Zhou, H. et al. Hybrid nanoflower-based electrochemical lateral flow immunoassay for Escherichia coli O157 detection. Microchim Acta 191, 453 (2024). https://doi.org/10.1007/s00604-024-06513-y

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