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A glassy carbon electrode modified with reduced graphene oxide and gold nanoparticles for electrochemical aptasensing of lipopolysaccharides from Escherichia coli bacteria

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Abstract

An electrochemical aptasensor is described for the voltammetric determination of lipopolysaccharide (LPS) from Escherichia coli 055:B5. Aptamer chains were immobilized on the surface of a glassy carbon electrode (GCE) via reduced graphene oxide and gold nanoparticles (RGO/AuNPs). Fast Fourier transform infrared, X-ray diffraction and transmission electron microscopy were used to characterize the nanomaterials. Cyclic voltammetry, square wave voltammetry and electrochemical impedance spectroscopy were used to characterize the modified GCE. The results show that the modified electrode has a good selectivity for LPS over other biomolecules. The hexacyanoferrate redox system, typically operated at around 0.3 V (vs. Ag/AgCl) is used as an electrochemical probe. The detection limit is 30 fg·mL−1. To decrease the electrochemical potential for detection of LPS, Mg/carbon quantum dots were used as redox active media. They decrease the detection potentialto 0 V and the detection of limit (LOD) to 1 fg·mL−1. The electrode was successfully used to analyze serum of patients and healthy persons.

Schematic representation of the modification of reduced graphene oxide gold nanoparticles with aptamer chains to immobilize on the glassy carbon electrode surface for electrochemical detection of lipopolysaccharides.

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Authors and Affiliations

  1. Protein Research Center, Shahid Beheshti University, Tehran, GC, 1983963113, Iran

    Mehrab Pourmadadi, Javad Shabani Shayeh & Meisam Omidi

  2. Department of Life Science Engineering, Faculty of New Science and Technologies, University of Tehran, Tehran, 1417466191, Iran

    Fatemeh Yazdian

  3. Pediatric Infections Research Center, Research Institute for Children‘s Health, Shahid Beheshti University of Medical Sciences, Tehran, 19839-63113, Iran

    Masoud Alebouyeh

  4. Marquette University School of Dentistry, Milwaukee, WI, 53233, USA

    Lobat Tayebi

Authors
  1. Mehrab Pourmadadi
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  2. Javad Shabani Shayeh
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  3. Meisam Omidi
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  4. Fatemeh Yazdian
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  5. Masoud Alebouyeh
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  6. Lobat Tayebi
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Correspondence to Javad Shabani Shayeh.

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Pourmadadi, M., Shayeh, J.S., Omidi, M. et al. A glassy carbon electrode modified with reduced graphene oxide and gold nanoparticles for electrochemical aptasensing of lipopolysaccharides from Escherichia coli bacteria. Microchim Acta 186, 787 (2019). https://doi.org/10.1007/s00604-019-3957-9

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  • DOI: https://doi.org/10.1007/s00604-019-3957-9

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