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Synergistic effect of organic-inorganic hybrid monomer and polyhedral oligomeric silsesquioxanes in a boronate affinity monolithic capillary/chip for enrichment of glycoproteins

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Abstract

A boronate affinity monolith with improved affinity and selectivity for glycoproteins was prepared starting from two monomers. The first is 3-aminopropyltriethoxysilane-methacrylic acid (APTES-MAA), and the other is a polyhedral oligomeric silsesquioxane (POSS) monomer. In the next step, 3-(acrylamido)benzeneboronic acid was adopted as boronate affinity ligand, and ethylene glycol dimethacrylate as the crosslinker, and iso-propanol and octanol as binary porogens. The synergistic effect of APTES-MAA and POSS warrants good affinity and selectivity for glycoproteins, which results in a number of attractive features including (a) a wide operation pH range (from 5 to 8); (b) higher enrichment factors ranging from 19.3 to 20.6; (c) greater recoveries of glycoproteins between 95.8 and 107.1%; (d) lower relative standard deviations of ≤4.2%. Compared to the corresponding APTES-MAA/POSS-free monolith, the new boronate material had 1.7-fold increased glycoprotein recovery from complex samples. Glycoproteins in 500-fold diluted serum samples can be enriched by the boronate monolith.

Schematic representation of the preparation of 3-aminopropyltriethoxysilane-methacrylic acid/polyhedral oligomeric silsesquioxanes boronate affinity monolith. This sorbent exhibits high selectivity and wide pH operation range for capturing glycopeptides.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (Grant No. 21775109).

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Authors and Affiliations

  1. Tian** Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tian** Medical University, Tian**, 300070, China

    Yan-Feng Shen, Fang-Fang Yuan, **n-Yu Liu, Yan-** Huang & Zhao-Sheng Liu

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  1. Yan-Feng Shen
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  2. Fang-Fang Yuan
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  3. **n-Yu Liu
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  4. Yan-** Huang
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  5. Zhao-Sheng Liu
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Correspondence to Yan-** Huang or Zhao-Sheng Liu.

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Shen, YF., Yuan, FF., Liu, XY. et al. Synergistic effect of organic-inorganic hybrid monomer and polyhedral oligomeric silsesquioxanes in a boronate affinity monolithic capillary/chip for enrichment of glycoproteins. Microchim Acta 186, 812 (2019). https://doi.org/10.1007/s00604-019-3938-z

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  • DOI: https://doi.org/10.1007/s00604-019-3938-z

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